摘要
为了提高牛细管冻精冻前和冻后的精子活力,改良冻精受胎率,试验用涂抹发情母牛阴道黏液刺激种公牛、在日粮中添加1%中草药添加剂、用不同温度和时间解冻牛冷冻精液的方法测定冻前和冻后精液品质指标(精液量、精子活力、精子密度、生产冻精数、精子复苏率),筛选可提高西门塔尔牛种公牛冷冻精液品质的条件。结果表明:与未涂抹发情母牛阴道黏液种公牛比较,涂抹发情母牛阴道黏液的种公牛鲜精精子活力和冻后精子活力及牛均生产冻精数均显著提高(P<0.05);鲜精精子密度和牛均新鲜精液量有所提高,但差异不显著(P>0.05)。种公牛日粮中添加中草药添加剂可显著提高鲜精精子活力、冻后精子活力和精子密度(P<0.05),精子复苏率也明显提高,但差异不显著(P>0.05)。90℃解冻3~4 s、80℃解冻4~5 s、70℃解冻4~6 s, 60℃解冻8~9 s, 50℃解冻20~30 s时精子活力较高,其中以70℃解冻牛细管冻精4~6 s、80℃解冻4~5 s时精子活力最高且较为稳定。说明应用发情母牛阴道黏液刺激、饲喂中草药添加剂及合理的牛冻精解冻温度和时间可提高牛冻精精子活力。
In order to improve the sperm motility before and after freezing of bovine straw frozen semen and improve the fertilization rate of frozen semen, semen quality indicators(semen volume, sperm motility, sperm density, number of frozen sperm produced, sperm recovery rate) before and after freezing were measured by stimulating the bulls with vaginal mucus from estrus cows, adding 1% Chinese herbal medicine additives in the diet, and thawing frozen semen at different temperatures and times, and the conditions that can improve the quality of frozen semen of Simmental bulls were screened. The results showed that the sperm motility of fresh sperm and frozen sperm, and the number of frozen semen available for production in the stud bulls smeared with vaginal mucus from estrus cows were significantly improved compared with stud bulls unsmeared with vaginal mucus from estrus cows(P<0.05),and the sperm density and semen volume of fresh sperm of each cattle were increased, but the differences were not significant(P>0.05). Adding Chinese herbal supplement to the diets of breeding bulls significantly improved the fresh sperm motility, frozen sperm motility and sperm density of the bulls(P<0.05), and the sperm recovery rate was also significantly improved, but the difference was not significant(P>0.05). The sperm motility was higher when thawed at 90 ℃ for 3-4 s, at 80 ℃ for 4-5 s, at 70 ℃ for 4-6 s, at 60 ℃ for 8-9 s, and at 50 ℃ for 20-30 s. The sperm motility was the highest and stable when the bovine frozen semen was thawed at 70 ℃ for 4-6 s and 80 ℃ for 4-5 s. The results indicated that the application of vaginal mucus stimulation of estrus cows, feeding of Chinese herbal medicine additives and reasonable thawing temperature and time of frozen bovine semen could improve the sperm motility of frozen bovine semen.
作者
姚奕蕾
孔炜
顾慧慧
YAO Yilei;KONG Wei;GU Huihui(Animal Disease Prevention and Control Center of Gansu Province,Lanzhou 730046,China;Livestock Breeding Center of Gansu Province,Wuwei 733000,China;Animal Husbandly Technology Extension Station of Gansu Province,Lanzhou 730030,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2022年第14期62-66,共5页
Heilongjiang Animal Science And veterinary Medicine
关键词
西门塔尔牛
发情母牛
阴道黏液
中草药添加剂
冻精
解冻温度
解冻时间
精子活力
Simmental cattle
rutting cows
vaginal mucus
Chinese herbal medicine additives
frozen sperm
thawing temperature
thawing time
sperm motility
作者简介
姚奕蕾(1983-),女,高级兽医师,本科,研究方向为动物疫病监测,25621574@qq.com;通信作者:顾慧慧(1996—),女,助理畜牧师,硕士,研究方向为肉牛遗传改良,guhuihui3053@163.com.
