摘要
目的 探究异氟醚对膀胱癌5637细胞恶性生物学行为及化疗敏感性的影响.方法 单独或联合给予异氟醚及顺铂处理膀胱癌5637细胞,将细胞随机分为4组:对照组、异氟醚组、顺铂组和异氟醚+顺铂联合处理组.BrdU染色检测BrdU阳性细胞数;流式细胞仪检测细胞凋亡率和细胞周期分布;Transwell检测侵袭细胞数;划痕愈合实验检测划痕愈合率;Western印迹检测Ki67、PCNA、Bax、Bcl-2、cleaved caspase-3和caspase-3蛋白表达;结果 与顺铂组相比较,异氟醚+顺铂联合处理组BrdU阳性细胞数和Ki67、PCNA蛋白表达降低,G0/G1期比率和S期细胞数降低,G2/M期比率升高,细胞凋亡率和Bax/Bcl-2、cleaved caspase-3/caspase-3比值升高,侵袭细胞数、划痕愈合率降低,均有显著性差异(P<0.05).结论 异氟醚可通过抑制细胞增殖、侵袭、迁移,诱导细胞凋亡,提高5637细胞对顺铂的敏感性.
Objective To investigate the effect of isoflurane on malignant phenotype and chemotherapy sensitivity of bladder cancer cell line 5637.Methods The 5637 bladder cancer cells were divided into groups as follows:control group,isoflurane group,cisplatin group and isoflurane+cisplatin group.The number of BrdU positive cells was determined by BrdU staining.The cell apoptosis and cell cycle were measured by flow cytometry.The number of invasive cells were measured by transwell assay.The scratch healing rate was measured by wound healing experiment.The expression levels of Ki67,PCNA,Bax,Bel-2,cleaved caspase-3 and caspase-3 proteins were detected by Western blotting.Results The number of BrdU positive cells,the expression levels of Ki67 and PCNA proteins,the G0/G1,phase ratio,the number of S phase cells were decreased significantly in the isoflurane+cisplatin group compared to the cisplatin group,while the G2/M phase ratio was increased significantly(P<0.05).The number of invasive cells and the scratch healing rate were decreased significantly in the isoflurane+cisplatin group when compared to the cisplatin group(P<0.05).The apoptosis rate,the Bax/Bcl-2 ratio,and the cleaved caspase-3/caspase-3 ratio were increased significantly in the isoflurane+cisplatin group compared to the cisplatin group(P<0.05)Conclusion Isoflurane can induce apoptosis by inhibiting cell proliferation,invasion and migration,and enhance the sensitivity of 5637 cells to cisplatin.
作者
田青川
杨晓春
张玉杰
TIAN Qingchuan;YANG Xiaochun;ZHANG Yujie(Department of Anesthesiology,Qinhuangdao First Hospital,Qinhuangdao,Hebei,066099,China)
出处
《医学分子生物学杂志》
CAS
2022年第4期295-299,共5页
Journal of Medical Molecular Biology
基金
秦皇岛市科学技术研究与发展计划项目(No.201805A116)。
关键词
膀胱癌
异氟醚
顺铂
化疗敏感性
bladder cancer
isoflurane
cisplatin
chemotherapeutic sensitivity
作者简介
通讯作者:田青川,电话:15176525377,E-mail:hpojj5646@163.com。
