摘要
建立液相色谱-质谱/质谱法测定茶叶中灭螨醌和羟基灭螨醌的分析方法。参照《出口食品中灭螨醌和羟基灭螨醌残留量的测定液相色谱-质谱/质谱法》(SN/T 4066—2014)前处理,采用正离子源模式和多反应监测模式进行检测,外标法定量。结果表明,灭螨醌和羟基灭螨醌在2~50 ng·mL^(-1)线性良好,相关系数为0.99995和1.00000,灭螨醌的定量限为4μg·kg^(-1),羟基灭螨醌的定量限为2μg·kg^(-1)。当加标质量浓度为2.5~40.0μg·kg^(-1)时,回收率为81.6%~97.4%,相对标准偏差为1.92%~2.39%(n=6)。该方法满足茶叶中灭螨醌和羟基灭螨醌残留量测定的要求。
A method for the determination of acequinocyl and acequinocyl hydroxy in tea by liquid chromatography-mass spectrometry/mass spectrometry was established.According to SN/T 4066—2014 pretreatment,positive ion source mode and multi reaction monitoring mode are used for detection,and external standard method is used for quantification.The results showed that acequinocyl and acequinocyl hydroxy has good linearity in the concentration range of 2~50 ng·mL^(-1),the quantitative limit of acequinocyl is 4μg·kg^(-1),the quantitative limit of acequinocyl hydroxy is 2μg·kg^(-1).The correlation coefficient of acequinocyl and acequinocyl hydroxy was 0.99995 and 1.00000,the recovery rates were 81.6%~97.4%with the relative standard deviation between 1.92%~2.39%(n=6)at the concentration of 2.5~40μg·kg^(-1).The method can meet the requirements for the determination of acequinocyl and acequinocyl hydroxy residues in tea.
作者
李苗
代菲
金萍
丁洪流
秦孟姣
LI Miao;DAI Fei;JIN Ping;DING Hongliu;QIN Mengjiao(Suzhou Institute of Product Quality Supervision and Inspection,Suzhou 215104,China;SCIEX Analytical Instrument Trading Co.,Ltd.,Shanghai 200335,China)
出处
《现代食品》
2022年第11期189-192,共4页
Modern Food
基金
苏州市科技局课题“烟碱类农药纳米抗体制备及其在食品农产品高灵敏高通量快速检测技术应用研究”(SS202038)。
关键词
茶叶
灭螨醌
羟基灭螨醌
tea
acequinocyl
acequinocyl hydroxy
作者简介
李苗(1987-),女,本科,工程师,研究方向为食品安全与质量控制;通信作者:丁洪流(1979-),女,博士,正高级工程师,研究方向为食品安全与质量控制。E-mail:hollior@163.com。
