摘要
This study explored the usefulness of two-dimensional shear wave elastography(2D-SWE)in the early assessment of corpora cavernosa fibrosis(CCF).New Zealand male rabbits were randomly assigned to an experimental group or a control group.Recombinant human transforming growth factor beta 1(TGF-β1)was injected into the dorsal penis tissue of rabbits in the experimental group.Conventional ultrasound and 2D-SWE examinations were performed before and 20 days after injection.Penile histological analysis was performed by hematoxylin–eosin staining,sirius red staining,and immunohistochemistry.Measurement of 2D-SWE examination results was performed using shear wave elastography quantitative measurement(SWQ).Histological analysis outcomes were the proportion of smooth muscle cells(SMCs),collagen fibers(CFs),collagen type I(Col I),and collagen type III(Col III),as well as the SMCs/CFs ratio,measured by sirius red staining.Other histological analysis outcomes were the positive area proportion(PAP)of TGF-β1(PAPT),fibronectin(PAPF),and Col III(PAPC),measured by immunohistochemistry.After recombinant human TGF-β1 injection,SWQ was higher in the experimental group than that in the control group(P<0.001);however,there were no differences in conventional ultrasound results.There were significant differences in histological outcomes between the two groups(all P<0.05).These results indicated that 2D-SWE was superior for identifying early histological changes in CCF.
基金
This study was supported by grants from the Scientific Research Foundation of Shanghai Pudong Hospital,Fudan University Pudong Medical Center(No.YJRCJJ201804)
the Outstanding Clinical Discipline Project of Shanghai Pudong(NO.PWYgy-2018-08)
the Scientific Research Foundation of Shanghai Key Laboratory of Vascular Lesions Regulation and Remodeling(NO.zdsys202008).
作者简介
Li Yu,These authors contributed equally to this work;Wan-Ting Rao,These authors contributed equally to this work;Correspondence:Jin-Fang Xing(xingshi70188@163.com)。
