摘要
目的探讨G2/S期应答相关蛋白1(GTSE1)在前列腺癌(PCa)组织的表达及其对前列腺癌细胞转移的调控作用。方法对GEPIA、UALCAN和FireBrowse数据库进行分析,明确GTSE1的表达与前列腺癌患者临床指标及转移的相关性,并进一步分析该基因的共表达基因及可能的作用通路。通过划痕实验和Transwell侵袭实验检测GTSE1对前列腺癌细胞体外迁移与侵袭能力的影响;Western blotting实验分析GTSE1对上皮间质转化(EMT)分子标记物的影响。结果在前列腺癌组织中,尤其是转移性的前列腺癌组织,GTSE1的表达显著升高,并与患者疾病进展和淋巴结转移密切相关。过表达GTSE1可增强前列腺癌细胞的迁移和侵袭能力,并促进其发生EMT;而在敲低GTSE1的前列腺癌细胞表现出相反的作用。GTSE1可能通过作用于前列腺癌细胞的微管,在TPX2、TOP2A和CENPF等分子的协同下驱动前列腺癌的EMT和转移。结论GTSE1在前列腺癌组织中高表达,并可促进前列腺癌细胞的侵袭、迁移能力,可能成为转移性前列腺癌治疗的新型分子靶点。
Objective To investigate the expression of G2 and S phase-expressed-1(GTSE1)in prostate cancer(PCa)tissues and its regulation in PCa metastasis.Methods GEPIA,UALCAN and FireBrowse databases were analyzed to determine the correlation between GTSE1 expression and clinical indexes and metastasis in patients with PCa.The co-expression genes and possible pathways of GTSE1 were further analyzed.Wound healing and transwell invasion assays were used to detect the effect of GTSE1 on the migration and invasion of PCa cells in vitro.Western blotting assay was used to analyze the effects of GTSE1 on epithelial-to-mesenchymal transition(EMT)markers.Results The expression of GTSE1 was significantly increased in PCa tissues,especially in metastatic PCa,and was closely related to the disease progression and lymph node metastasis.Overexpression of GTSE1 enhanced the ability of migration and invasion of PCa cells,and promoted the formation of EMT,which showed the opposite effects with GTSE1 knockdown.GTSE1 may act on the microtubules of PCa cells and cooperate with TPX2,TOP2A and CENPF to drive EMT and metastasis of PCa.Conclusion GTSE1 is highly expressed in PCa tissues and can promote the invasion and migration of PCa cells,which may be developed as a new molecular target for the treatment of metastatic PCa.
作者
祝炜安
赖文杰
李小娟
郑嘉裕
包琨
蔡有弟
张丽媚
黄炯煅
温星桥
Zhu Wei'an;Lai Wenjie;Li Xiaojuan;Zheng Jiayu;Bao Kun;Cai Youdi;Zhang Limei;Huang Jiongduan;Wen Xingqiao(Department of Urology,the Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China;Department of Health Care,Shenzhen Hospital,Southern Medical University,Shenzhen 518101,China)
出处
《中华腔镜泌尿外科杂志（电子版）》
2022年第3期270-275,共6页
Chinese Journal of Endourology(Electronic Edition)
基金
国家自然科学基金面上项目(81874095,82072820)
广东省基础与应用基础研究基金联合基金重点项目(2019B1515120007)。
作者简介
通信作者:温星桥,Email:wenxq@mail.sysu.edu.cn。
