摘要
目的:为明确不同贮藏温度对玉米种子活力以及幼苗相关基因的影响,选取不同温度贮藏的玉米种子,对种子的发芽能力以及幼苗相关基因进行分析。方法:将常温(25~30℃)和低温(0~4℃)贮藏的种子在人工气候箱内进行标准发芽试验,分别在实验后的第3天和第7天计算发芽势和发芽率并通过实验数据计算发芽指数和活力指数。在第7天后分别取常温和低温幼苗进行转录组测序,将筛选出的差异基因在相应的数据库中进行对比(KOG、GO、KEGG),分析处理差异基因的功能和参与的调控路径。结果:低温贮藏的玉米种子在发芽势、发芽率、发芽指数、活力指数上显著高于自然贮藏的对照种子。在转录组测序和数据分析中,低温贮藏的玉米种子与自然贮藏的玉米种子处理间筛选出4个差异基因,包含3个上调基因和1个下调基因。在GO数据分析中4个差异基因分别涉及到应激反应等生理或生化过程;在KEGG代谢通路中4个差异基因显著富集植物-病原互作、嘌呤代谢、N-聚糖生物合成代谢通路中。结论:在自然贮藏和低温贮藏条件下,植物-病原互作、N-聚糖生物合成、嘌呤代谢相关基因在玉米种子活力中发挥重要作用。
Objective:To determine the effects of different storage temperatures on maize seed vigor and seedling-related genes.Methods:Standard germination experiments were carried out in artificial climate box under 25~30℃and 0~4℃storage conditions.Germination potential and germination rate were calculated on the 3rd and 7th day after experiment respectively,and germination index and vigor index were calculated through experimental data.The transcriptome was sequenced after the 7th day at room temperature and low temperature,and the differential genes were compared in the corresponding databases,KOG,GO and KEGG.Results:The germination vigor,germination rate,germination index and vigor index of maize seeds stored at low temperature were significantly higher than those of control seeds stored at low temperature.In transcriptome sequencing and data analysis,four differential genes,including three up-regulated genes and one down-regulated gene,were screened between cold-stored and natural-stored maize seeds.In GO data analysis,four different genes were involved in physiological or biochemical processes such as anti-stimulation.In the KEGG metabolic pathway,four differential genes were significantly enriched in plant-pathogen interaction,purine metabolism,and N-glycan biosynthesis pathways.Conclusion:Plant-pathogen interaction,N-glycan biosynthesis and purine metabolism related genes play an important role in maize seed vigor under natural and low-temperature storage conditions.
作者
姚文豪
陈志英
王杰
徐魏
李文阳
YAO Wenhao;CHEN Zhiying;WANG Jie;XU Wei;LI Wenyang(College of Agriculture,Anhui Science and Technology University,Fengyang 233100,China)
出处
《安徽科技学院学报》
2022年第2期42-47,共6页
Journal of Anhui Science and Technology University
基金
国家自然科学基金(31501271)
安徽省高校自然科学研究重大项目(KJ2020ZD010)
安徽省财政厅成果转化项目(2021ZH015)
国家级大学生创新创业训练计划项目(202010879112)。
关键词
玉米
低温贮藏
幼苗生长
转录组分析
差异基因
Corn
Low temperature storage
Seedling growth
Transcriptome analysis
Differential gene
作者简介
姚文豪(2000-),男,安徽淮南人,本科生,主要从事作物高产优质栽培研究。;通信作者:李文阳,教授,E-mail:liwy@ahstu.edu.cn。
