摘要
目的分析HIV感染者单个核细胞(PBMCs)内HIV DNA和细胞相关RNA(CA RNA)在ART过程中的水平变化特点,并结合临床资料以及炎症因子水平分析二者水平变化不一致的可能原因。方法纳入2009年11月至2013年7月于解放军总医院第五医学中心确诊并接受随访满5年的27例HIV感染者作为研究对象,采集外周静脉血分离出PBMCs后冷冻保存,选取ART 0、1、3、5年的PBMCs标本采用荧光定量PCR的方法检测PBMCs内HIV DNA和CA RNA,并结合HIV感染者的临床资料以及炎症因子进行分析。结果经过5年ART,所有HIV感染者的病毒载量均降至检测水平以下,HIV DNA和CA RNA水平M(P_(25),P_(75))分别为3.03(2.74,3.33)、3.11(2.63,3.49)log_(10)拷贝/10^(6)PBMCs,分别与基线水平进行比较,差异均有统计学意义(P均<0.05);27例HIV感染者中有8例HIV DNA和CA RNA的水平变化不一致,在ART 3年时CA RNA的水平会明显升高,与HIV DNA的水平变化趋势相反;临床资料显示这8例HIV感染者在ART 3年时均发生了其他病原感染,并伴随着炎症因子水平的升高。结论合并其他病原感染在一定程度上可以激活潜伏的病毒库,CA RNA水平会升高,但对HIV DNA水平的影响很小。
Objective To analyze the changes in the levels of HIV DNA and RNA in peripheral blood mononuclear cells(PBMCs)of HIV-infected patients during long-term antiretroviral therapy(ART),and to analyze the possible reasons for the inconsistent changes in the levels of both,taking into account the clinical data and the levels of inflammatory factors.Methods 27 HIV-infected patients who were diagnosed in the Fifth Medical Centre of the PLA General Hospital and followed up for 5 years from November 2009 to July 2013 were included as the research subjects.Their peripheral venous blood was collected to isolate PBMCs and then cryopreserved.PBMCs specimens from ART 0,1,3,and 5 years were selected to detect the levels of HIV DNA and CA RNA in PBMCs by fluorescence quantitative PCR,and analyzed together with clinical data and inflammatory factors of HIV-infected patients.Results After 5 years of ART,the viral load in all HIV-infected individuals dropped below the detection level,and the HIV DNA and CA RNA levels were 3.03(2.74-3.33)and 3.11(2.63-3.49)log_(10) copies/10^(6) PBMCs,respectively,which were comparable to the baseline levels,and the differences were statistically significant(all P<0.05).8 of the 27 HIV-infected patients had inconsistent changes in the levels of HIV DNA and CA RNA,and 3 years of ART,the level of CA RNA increased significantly at the time of ART which was opposite to the trend of the level of HIV DNA.Clinical data showed that the 8 HIV-infected patients had other pathogenic infections at 3 years of ART and the levels of inflammatory factors increased.Conclusions Co-infection with other pathogens can activate the latent virus reservoir to a certain extent.The level of CA RNA will increase but it has little influence on HIV DNA level.
作者
张晴
高林
郭晓燕
郭云天
焦艳梅
王福生
ZHANG Qing;GAO Lin;GUO Xiaoyan;GUO Yuntian;JIAO Yanmei;WANG Fusheng(Graduate School of Bengbu Medical College,Bengbu 233000,Anhui,China;Department of Infectious Diseases,Fifth Medical Centre of Chinese PLA General Hospital,Beijing 100039)
出处
《中国艾滋病性病》
CAS
CSCD
北大核心
2022年第6期647-651,共5页
Chinese Journal of Aids & STD
基金
“十三五”国家科技重大专项(2018ZX10302104-002-001)
国家自然科学基金(8217060332,82171732)。
作者简介
第一作者:张晴(1995-),女,安徽省宿州市人,硕士在读,从事艾滋病的临床免疫学研究。Email:zq18949346390@163.com;通信作者:焦艳梅,Email:jiaoyanmei@sina.com;通信作者:王福生,Email:fswang302@163.com。
