摘要
Vitamin E is one of the most widely used vitamins.In the classical commercial synthesis of vitamin E(a-tocopherol),the chemical synthesis of isophytol is the key technical barrier.Here,we establish a new process for isophytol synthesis from microbial fermented farnesene.To achieve an efficient pathway for farnesene production,Saccharomyces cerevisiae was selected as the host strain.First,b-farnesene synthase genes from different sources were screened,and through protein engineering and system metabolic engineering.
基金
This work was financially supported by the National Key R&D Program of China(2018YFA0900400)
the National Natural Science Foundation of China(31670090).We also acknowledge Professor Zihe Liu fromthe Beijing University of Chemical Technology for kindly providing the pCas and KlURA3 plasmid.
作者简介
Correspondence:Tiangang Liu,liutg@whu.edu.cn。
