摘要
从活性污泥中筛选出以丙硫菌唑为唯一碳源的降解菌株,对该菌株进行形态学、生理和生化特征结合16Sr DNA基因序列鉴定为假单胞菌属绿脓杆菌,命名为Pseudomon asaeruginosaW-313。通过响应面法对菌株W-313降解丙硫菌唑的条件进行优化,初始pH值为7.40,温度为32℃,葡糖糖含量为0.60%时,W-313降解丙硫菌唑效果最佳,48h降解率可达65.12%,与实际情况下基本吻合。研究结果为利用环境微生物降解丙硫菌唑及其代谢物进而有效规避残留农药污染提供新理论依据。
A degrading strain with prothioconazole as the sole carbon source was selected from the activated sludge, and the strain was subjected to morphological, physiological and biochemical characteristics combined with 16S rDNA gene sequence. As a result, it was identified as pseudomonas aeruginosa, named as Pseudomonas aeruginosa W-313. The conditions for strain W-313 to degrade prothioconazole were optimized by response surface methodology. When the initial pH was 7.40, the temperature was 32 ℃, and the glucose content was 0.60%, W-313 had the best degradation effect on prothioconazole with the degradation rate of 65.12% in 48 h, which was basically consistent with the actual situation. The results of this study provide a new theoretical basis for using environmental microorganisms to degrade prothioconazole and its main metabolite,which can effectively avoid residual pesticide pollution.
作者
林凤翔
叶壮
胡芃
王莹
赵素霞
魏东
施艳红
LIN Fengxiang;YE Zhuang;HU Peng;WANG Ying;ZHAO Suxia;WEI Dong;SHI Yanhong(School of Resources and Environment,Anhui Agricultural University,Hefei 230036)
出处
《安徽农业大学学报》
CAS
CSCD
2022年第2期279-285,共7页
Journal of Anhui Agricultural University
基金
安徽省科技重大专项(17030701050)
国家级大学生创新创业项目(202010364067)共同资助。
关键词
丙硫菌唑
降解菌
生物降解
响应面设计
prothioconazole
degradation strains
biodegradation
response surface design
作者简介
林凤翔,硕士研究生。E-mail:linfid999@163.com;通信作者:施艳红,博士,教授。E-mail:shiyh@ahau.edu.cn。
