摘要
线虫是一种危害极大的植物病原生物,给农业生产带来巨大危害.采用平板稀释法从轿子山土样和白云鄂博稀土矿样品中分离放线菌并鉴定,利用24孔板液体模型筛选具有杀秀丽隐杆线虫的放线菌.结果表明,分离的257株放线菌隶属37属、24科,有5株菌的发酵液对2龄秀丽隐杆线虫的致死率高于70%,经16S rDNA分析鉴定5株菌KC 97,KC 107,KC 113,KC 36,KC 240分别为Pseudonocardia seranimata,Micromonospora echinofusca,Pseudonocardia rhizophila,Micromonospora peucetia,Saccharothrix espanaensis.获得的活性菌株为线虫的生物防治提供了丰富的微生物资源.
Nematode is a plant pathogenic organism with great harm to farming.Actinomycetes from Jiaozi Mountain and Bayanobo rare earth mine samples were isolated and identified by plate dilution method and screened activity for killing Caenorhabditis elegans by 24 well plate liquid model.The results showed that 257 representative isolates were obtained belonging to 37 genera and 24 families,among which 5 strains fermentation has high nematocidal activity against C.elegans with mortalities above 70%.Based on 16S rDNA sequence analysis,5 Actinomycetes of KC 97,KC107,KC113,KC36 and KC240 were identified as Pseudonocardia seranimata,Micromonospora echinofusca,Pseudonocardia rhizophila,Micromonospora peucetia,Saccharothrix espanaensis.The active Actinomycete strains are good resource for researching biological control of plant-parasitic nematode disease.
作者
朱国兴
焦钰
吴雅杰
樊炳君
叶秀娟
曹艳茹
ZHU Guoxing;JIAO Yu;WU Yajie;FAN Bingjun;YE Xiujuan;CAO Yanru(School of Agriculture and Life Sciences,Kunming University,Kunming,Yunnan,China 650214;Scientific and Technological Innovation Team for Agricultural Resource Utilization Kunming University,Kunming,Yunnan,China 650214;Engineering Research Center for Exploitation and Utilization of Leech Resources,Kunming,Yunnan,China 650214)
出处
《昆明学院学报》
2022年第3期93-99,120,共8页
Journal of Kunming University
基金
云南省地方本科高校(部分)基础研究联合专项资金项目(2018FH001-003)
云南省教育厅科学研究基金项目(2021Y733)
云南省“万人计划”青年拔尖人才资助项目(YNWR-QNBJ-2018-011)
省部共建云南生物资源保护与利用国家重点实验室开放课题(2019KF005)
国家自然科学基金项目(31660002).
关键词
秀丽隐杆线虫
放线菌
筛选
鉴定
Caenorhabditis elegans
Actinomycetes
screening
identification
作者简介
朱国兴(1995—),女,安徽亳州人,硕士研究生,主要从事微生物资源利用及植物保护研究;通信作者:曹艳茹(1983—),女,内蒙古乌海人,教授,博士,主要从事微生物研究,E-mail:yanrucao3@aliyun.com.
