摘要
目的:制备一种快速检测流行性乙型脑炎病毒(Japanese encephalitis virus,JEV)IgG抗体的试纸条,为其现场检测提供新的工具。方法:采用免疫层析技术,以胶体金标记的金黄色葡萄球菌蛋白A为标记物,将JEV特异性原核表达抗原JEVAg45固化于硝酸纤维素膜上作为检测抗原,以JEV抗体阴性的人IgG抗体为质控,制备胶体金快检试纸条,并对该试纸条的敏感性、特异性、稳定性及临床应用进行评估。结果:该试纸条可检测按1∶10~1∶640稀释的JEV多克隆抗体,且同批次试纸条的检测结果一致。该试纸条与其他13种相近虫媒病毒无交叉反应,与免疫荧光法检测结果的符合率为100%。结论:该试纸条具有较高的敏感性、特异性和稳定性,为基层单位快速筛查流行性乙型脑炎提供了新的技术手段。
Objective To develop a test strip for on-site detection of the IgG antibody to Japanese encephalitis virus(JEV).Methods A colloidal gold rapid test strip was prepared with immunochromatographic techniques,colloidal gold-labeled staphylococcus aureus protein A as the marker,JEV-specific prokaryotic expression antigen JEVAg45 cured on a nitrocellulose membrane as the detection antigen and JEV antibody-negative human IgG antibody as the quality control material.Then the sensitivity,specificity,stability and clinical application of the test strip prepared were evaluated.Results The test strip could detect JEV polyclonal antibodies diluted from 1∶10 to 1∶640,and the same batch of strips gained consistent results.The strip prepared showed no cross-reactivity with 13 other similar arboviruses,and the compliance rate with the results of immunofluo-rescence assay was 100%.Conclusion The test strip prepared has high sensitivity,specificity and stability,providing a new technical means for rapid screening of Japanese encephalitis(JE)in primary institutions.
作者
李春缘
梁克峰
任瑞文
陈迪佳
刘炅
刘军
陈月
田靖
LI Chun-yuan;LIANG Ke-feng;REN Rui-wen;CHEN Di-jia;LIU Jiong;LIU Jun;CHEN Yue;TIAN Jing(Center for Disease Control and Prevention of Southern Theater Command,Guangdong Arbovirus Diseases Emergency Technology Research Center,Guangzhou 510507,China)
出处
《医疗卫生装备》
CAS
2022年第5期29-32,共4页
Chinese Medical Equipment Journal
基金
全军后勤科研项目(A3702022003,17SAZ11)
广东省科技计划项目(2016A020219006,2014A020219007)
广东省医学科学技术研究基金项目(A2016062)。
关键词
JE
JEV
胶体金
免疫层析技术
IGG抗体
试纸条
Japanese encephalitis
Japanese encephalitis virus
colloidal gold
immunochromatographic technique
IgG antibody
test strip
作者简介
李春缘(1989-),女,硕士,研究实习员,主要从事病原检测及虫媒病毒监测方面的研究工作,E-mail:657724547@qq.com;通信作者:田靖,E-mail:tianjing1980s@126.com。
