摘要
目的了解湖南省湘西自治州黄牛泰勒虫感染率和遗传变异。方法2018年8月至2019年8月,从湘西自治州凤凰县、花垣县和保靖县采集184份黄牛血液样品,用基于泰勒虫属18S核糖体RNA(18S r RNA)基因的PCR方法对所有血液样品进行检测。将部分阳性产物基因序列与GenBank数据库中收录的相应虫株序列进行同源性比对,以卵形疟原虫18S rRNA基因作为外群构建种系发育树。结果共143份血液样品检测为泰勒虫阳性,平均检出率为77.7%。凤凰县、花垣县和保靖县黄牛血液样品均检出泰勒虫,检出率分别为85.0%、88.3%和61.0%;湘西黄牛和普通黄牛泰勒虫检出率分别为77.2%和79.5%(χ^(2)=0.08,P>0.05);舍饲牛群与散养牛群检出率分别为68.9%和89.7%(χ^(2)=22.36,P<0.01)。随机挑选18个PCR阳性样品进行测序与序列分析,发现所有样品与已知吕氏泰勒虫分离株同源性超过99.0%;系统进化树分析发现18个样品与吕氏泰勒虫聚为同一分支,但与其他虫株所属分支相隔较远。结论湖南省湘西地区黄牛泰勒虫感染率较高,吕氏泰勒虫可能为优势虫种。
Objective To investigate the prevalence and genetic variation of Theileria in yellow cattle in Xiangxi Autonomous Prefecture of Hunan Province.Methods A total of 184 blood specimens were collected from Fenghuang, Huanyuan and Baojing counties of Xiangxi Autonomous Prefecture during the period from August 2018 through August 2019, and were detect using PCR assay with the specific 18 S ribosomal rRNA(18 S r RNA) gene targeting Theileria. The gene sequences of positive specimens were aligned with the sequences recorded in GenBank, and a phylogenetic tree was created with Plasmodium ovale 18 S rRNA as an outgroup.Results A total of 143 blood samples were positive for Theileria, with a mean detection rate of 77.7%. Theileria was prevalent in the blood samples from yellow cattle in all three counties, with detection rates of 85.0% in Fenghuang County,88.3% in Huayuan County and 61.0% in Baojing County, respectively. There was no significant difference in the detection rate of Theileria between Xiangxi yellow cattle and normal yellow cattle(77.2% vs. 79.5%;χ^(2)= 0.08, P > 0.05), while the detection of Theileria was significantly lower in the housed yellow cattle than in free-range cattle(68.9% vs. 89.7%;χ^(2)= 22.36, P < 0.01). A total of 18 PCR positive samples were randomly selected for sequencing and analysis, and all samples showed more than 99.0%homology with T. luwenshuni isolates. Phylogenetic analysis showed that the 18 positive samples were clustered into the same branch with T. luwenshuni, but were far away from other isolates.Conclusions The prevalence of Theileria is high in yellow cattle from Xiangxi Autonomous Prefecture of Hunan Province, and T. luwenshuni may be the dominant parasite species.
作者
李晖
侯强红
汤国祥
吴阳祥
张大军
赵俊龙
宋金秋
LI Hui;HOU Qiang-hong;TANG Guo-xiang;WU Yang-xiang;ZHANG Da-jun;ZHAO Jun-long;SONG Jin-qiu(Xiangxi Vocational and Technical College,Jishou,Hunan 416000,China;Huaihua Vocational and Technical College,Hunan Province,China;Animal husbandry Workstation,in Heshan District,Yiyang City,Hunan Province,China;People,s Government of Lianglin Township in Fenghuang County,Hunan Province,China;College of Zoology and Medicine,Huazhong Agricultural University,Hubei Province,China)
出处
《中国血吸虫病防治杂志》
CAS
CSCD
北大核心
2022年第2期183-186,共4页
Chinese Journal of Schistosomiasis Control
基金
湘西特色现代农业科研团队项目(湘西职院[2020]15号)
湖南省教育厅项目(20C1810)
湖南省教育厅青年项目(21B0919)。
关键词
吕氏泰勒虫
18S核糖体RNA
分子流行病学
黄牛
湘西自治州
Theileria luwenshuni
18S ribosomal RNA
Molecular epidemiology
Yellow cattle
Xiangxi Autonomous Prefecture
作者简介
李晖,女,硕士,副教授。研究方向:动物疫病防控;通信作者:宋金秋,E-mail:64190208@qq.com,ORCID:0000-0003-0124-3955。
