摘要
采用HPLC法,用乙腈∶0.1%甲酸进行梯度洗脱,检测波长254 nm,流速1.0 mL/min,进样量10μL,同时测定凉粉草(Mesona chinensis Benth.)中的咖啡酸、异槲皮苷、紫云英苷和迷迭香酸4种成分含量,并进行系统适用性考察。结果显示,咖啡酸、异槲皮苷、紫云英苷、迷迭香酸分别在34.297~171.480μg/mL、11.172~55.860μg/mL、16.386~81.928μg/mL、13.394~66.970μg/mL浓度范围内线性关系良好,r分别为0.999 9、1.000 0、1.000 0、0.999 7;精密度、重复性、稳定性试验结果的RSD均小于3.00%;9次试验的4种成分相对应的平均回收率均大于95.00%,RSD均小于3.00%。
HPLC method was used with acetonitrile∶0.1% formic acid as gradient elution. The detection wavelength was 254 nm,the flow rate was 1.0 mL/min,and the injection volume was 10 μL. At the same time,the contents of four components in Mesona chinensis Benth.,including caffeic acid,isoquercitrin,astragaloside and rosmarinic acid were determined,and the applicability of the system was investigated. The results showed that caffeic acid,isoquercitrin,astragaloside and rosmarinic acid had a good linear relationships in the concentration ranges of 34.297~171.480 μg/mL,11.172~55.860 μg/mL,16.386~81.928 μg/mL and 13.394~66.970 μg/mL,respectively. The correlation coefficients were 0.999 9,1.000 0,1.000 0 and 0.999 7,respectively. RSD of precision,repeatability and stability test results were all less than 3.00%. The average recoveries of the four components in the nine tests were all greater than95.00% and RSD was less than 3.00%.
作者
莫颖华
冯白茹
毛一中
MO Ying-hua;FENG Bai-ru;MAO Yi-zhong(Huizhou Health Sciences Polytechnic,Huizhou 516025,Guangdong,China)
出处
《湖北农业科学》
2022年第9期114-117,共4页
Hubei Agricultural Sciences
基金
广东省普通高校特色创新类项目(2018GKTSCX097)。
作者简介
莫颖华(1982-),女,广东阳江人,讲师,硕士,主要从事中药及其制剂的质量分析研究,(电话)18319923684(电子信箱)89575870@qq.com。
