摘要
粒重稳定性是小麦稳产性的重要评价指标。为了发掘小麦在遭受虫害等减源逆境伤害时影响粒重稳定性的QTL,以小偃81和西农1376杂交衍生的重组自交系(RILs)群体为试验材料,设早播和晚播两个播期,对减源处理下该群体的粒长、粒宽和千粒重进行表型分析,同时计算减源处理与对照处理的表型比值,进而估测发育稳定性,并利用50K芯片构建的遗传连锁图谱进行QTL定位。结果表明,与对照处理相比,减源处理下RIL群体的粒长、粒宽和千粒重均显著下降。采用完备复合区间模型对不同处理的表型值、表型比值分别进行QTL定位,共检测到18个QTL,分布于2A、3A、3B、3D、4B、4D和5D染色体上,其中有8个QTL在减源处理和对照处理中均表达,Qgl.nwafu-5D.3和Qkgw.nwafu-5D均在早播对照处理和晚播减源处理中被检测到,平均可解释表型变异的8.73%和8.19%,为稳定QTL,且Qgl.nwafu-5D.3也在早播减源处理中被检测到,推测这2个稳定QTL可能为新发现的位点。控制表型比值的QTL大多与控制粒长、粒宽和千粒重的QTL区间重合,推测维持减源后粒重稳定性的QTL与控制粒长、粒宽和千粒重的位点紧密连锁或存在“一因多效”现象。
Grain weight stability is an important evaluation index of wheat yield stability.In order to find out the QTLs that affect the stability of grain weight in wheat under reduced source stress such as insect damage,the phenotypic and QTL analysis of grain length,grain width and 1000 grain weight were carried out in the RILs population derived from the cross between Xiaoyan 81 and Xinong 1376 under four environments of flag leaf removal as well as early sowing and late sowing.At the same time,the phenotypic ratios of source reduction treatment to control treatment was calculated,and the stability was estimated by the ratio to find the QTLs of ratios of phenotype,and the high-density genetic linkage map was constructed by using 50K SNP assay.The result showed that compared with the control treatment,the grain length,grain width and 1000-grain weight of RIL population were significantly decreased under source reduction treatment.And there were 18 QTLs distributed on 2A,3A,3B,3D,4B,4D and 5D chromosomes,8 QTLs controlling grain length,grain width and 1000 grain weight were expressed in source reduction treatment and control treatment,in which Qgl.nwafu-5D.3 and Qkgw.nwafu-5D may be the new locus,and Qgl.nwafu-5D.3 and Qkgw.nwafu-5D were stable QTLs with the average phenotypic vatiation of 8.73%and 8.19%,which were detected under control treatment at early sowing and source reduction treatment at late sowing,and Qgl.nwafu-5D.3 was also detected under source reduction treatment at early sowing.Most QTLs controlling phenotypic ratios coincide with those controlling grain length,grain width and 1000 grain weight,it is speculated that QTLs of maintaining the stability of grain weight after flag leaf removal are closely related to QTLs of grain length,grain width and 1000 grain weight or had“one cause,multiple effects”.
作者
周锋
杨虓
吕栋云
张传量
宋鹏博
姚俭昕
王鑫
王笑笑
孙道杰
ZHOU Feng;YANG Xiao;LÜDongyun;ZHANG Chuanliang;SONG Pengbo;YAO Jianxin;WANG Xin;WANG Xiaoxiao;SUN Daojie(Collge of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;College of Agriculture and Forestry,Longdong University,Qingyang,Gansu 745000,China)
出处
《麦类作物学报》
CAS
CSCD
北大核心
2022年第4期424-432,共9页
Journal of Triticeae Crops
基金
国家重点研发计划项目(2016YFD0101802)
西北农林科技大学长葛小麦试验示范站项目。
关键词
小麦
旗叶
粒形
粒重
稳定性
QTL
Wheat
Flag leaf
Grain morphology
Grain weight
Stability
QTL
作者简介
第一作者:周锋,E-mail:584822283@qq.com;通讯作者:孙道杰(E-mail:chinawheat@163.com)。
