摘要
鸡传染性支气管炎(IB)是目前对养禽业造成严重危害的疫病之一,其病原为传染性支气管炎病毒(IBV)。IBV由于其基因组的特点而易于发生变异和重组,从而产生新的变异株。为了解山西地区IBV的流行及变异情况,本研究采集山西省晋中市疑似发生呼吸道病毒感染的肉鸡组织病料样品,通过PCR鉴定、鸡胚接种及测序,分离鉴定到1株IBV,命名为CK/Shanxi-01/2021。在该分离株S1基因测序的基础上,与NCBI数据库中不同基因型IBV代表株进行序列比对,并构建系统进化树。遗传演化分析结果显示本研究所分离到的IBV为GI-19基因型。将CK/Shanxi-01/2021株的S1基因序列与IBV常用疫苗株H120、M41、H52、4/91及LDT3-A等进行序列比对分析,结果显示分离株与目前常用疫苗株核苷酸序列的同源性为78.1%~85.2%,氨基酸序列同源性为75.2%~78.4%,与以往GI-19基因型病毒株相比其S1蛋白高变区(HVR)出现了V68I、S120A、A271T、N282T、N291S等突变。由此可见,加强IBV流行病学监测,及时掌握目前流行的IBV基因型及其变异特点,对于IB疫情防控具有重要意义。本研究为山西省地区IB疫情防控提供了参考依据。
Chicken infectious bronchitis(IB)is one of the epidemic diseases that cause severe economic loss to poultry industry and induced by the infection of infectious bronchitis virus(IBV).IBV was prone to mutation and recombination due to its genomic characteristics,resulting in new antigenic variants.In order to understand the prevalence and variation of IBV in Shanxi province and provide reference for IB epidemic prevention and control in this area,tissues of a broiler flock suspected of respiratory virus infection were collected in Jinzhong city,Shanxi province.Through PCR identification,chicken embryo inoculation,and sequencing verification,an IBV strain was isolated and named CK/Shanxi-01/2021.On the basis of S1 gene sequencing,the sequence was compared with those of the representative IBV strains of different genotypes deposited in NCBI database,and the phylogenetic tree was constructed.The results of genetic evolution analysis showed that the IBV strain isolated in this study was belonged to GI-19 genotype.The sequence alignment of CK/Shanxi-01/2021 with common IBV vaccine strains H120,M41,H52,4/91,and LDT3-A showed that the nucleotide homology between the isolated strain and the current common vaccine strain was 78.1%-85.2%,and the amino acid homology was only 75.2%-78.4%.Compared with the sequences of GI-19 genotype strains,some new mutations,including V;I,S;A,A;T,N;T,and N;S,were identified in the S1 protein hypervariable region(HVR).Therefore,it is of great significance for the prevention and control of IB epidemic to strengthen the epidemiological monitoring of IBV and timely grasp the current epidemic IBV genotype and its variation characteristics.
作者
王旭贞
程宇
谭仕明
马海利
梁立滨
WANG Xu-zhen;CHENG Yu;TAN Shi-ming;Ma Hai-li;Liang Li-bin(Shanxi Animal Husbandry and Veterinary School,Taiyuan 030024,China;College of Veterinary Medicine,Shanxi Agricultural University,Taigu 030801,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2022年第3期320-324,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
山西省1331工程(20211331-13)
山西省重点研发计划重点项目(201703D211001)。
关键词
传染性支气管炎病毒
分离鉴定
S1基因
进化分析
infectious bronchitis virus
isolation and identification
S1 gene
phylogenetic analysis
作者简介
王旭贞(1971-),女,山西吕梁人,高级讲师,农学硕士,主要从事家禽养殖与疫病防控技术研究;通信作者:马海利,E-mail:mahaili1718@126.com;通信作者:梁立滨,lianglibin521@126.com。
