摘要
为揭示奶水牛分娩前后血浆瘦素(leptin,LEP)与部分氧化/抗氧化指标的变化特征及其意义,本试验采用动态监测方法,选择南宁市某牛场的水牛11头,分析产前1周至产后6周内血浆LEP、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)、总抗氧化能力(TAC)、丙二醛(MDA)的水平,产后逐日测定产奶量,分析其平均值、变异情况、动态变化特点及各指标间的相关性。结果显示,LEP平均值为1.26 ng/mL、CAT平均值为0.14 U/mL、GPX平均值为105.26 umol/L、SOD平均值为36.68 U/mL、TAC平均值为0.54(OD值)、MDA平均值为7.76 nmol/mL。变异系数以SOD最小(0.18)、GPX最大(0.66)。LEP在分娩当天达峰值,产后2周降到低谷,之后出现次波峰。TAC、CAT、GPX、SOD在产后3周降到最低,之后渐升,MDA产后1周降至低谷,产后3周接近峰值。产奶量2周内急增,之后趋于平稳。相关性分析为SOD-TAC,CAT-GPX间呈极显著正相关,MDA-SOD间呈极显著负相关。综上所述,LEP作为能量调节信号,水牛LEP在分娩与产后出现明显波动,且在产后2周内幅度最大,结合产奶量变化提示,此时能量调节力度最大。抗氧化水平在产后3周最低,因此,加强分娩与产后营养管理非常必要。这些数据可为奶水牛临床健康检查与管理提供参考。
To reveal the change characteristicsand significance of plasma leptin(lep)and partial oxidation/antioxidantlevels in milk buffalo around parturition,Dynamic monitoring method was adopted in this experiment,11 buffalo were selected from a farm in Nanning in this study.Plasmalevel of leptin(LEP),catalase(CAT),glutathione peroxidase(GPX),superoxide dismutase(SOD),total antioxidant capacity(TAC),and malondialdehyde(MDA)were weekly analyzedfrom 1 wk before to 6 wksafter parturition,milk yield was daily measured.The mean,variations,dynamic characteristics and correlation were analyzed.Results,the mean of LEP was 1.26 ng/mL,CAT 0.14 U/mL,GPX 105.26 umol/L,SOD 36.68 U/mL,TAC 0.54(OD value),and MDA 7.76 nmol/mL.The coefficient of variation was the lowest SOD(0.18)and the highest GPX(0.66).LEP reached its peak on the dayof parturition,decreased thento its trough 2 wkspostpartum,and then there's a secondary crest.TAC,CAT,GPX,and SOD decreased to its lowest level at 3 wks,and then gradually increased.MDA decreased to its lowest level at 1 wk pp,and approached its peak at 3 wkspostpartum.Milk production increased sharply within 2 wks and then leveled off.Thecorrelationanalysis showed that SOD-TAC,CAT-GPX had a very significant positive correlation,and MDA-SOD had a very significant negative correlation.In conclusion,as an energy regulation signal,LEP of buffalo fluctuates significantly during partum and postpartum,and the amplitude is the largest within 2 weeks postpartum.Combined with changes in milk yield,it indicates that energy regulation is the strongest at this time.The antioxidant level is the lowest at 3 weeks postpartum,therefore,it is necessary to strengthen the nutrition management during calving and postpartum.These data can provide reference for clinical health examination and management of milk buffalo.
作者
毕方方
唐阳国
方治山
何宝祥
杜玉兰
BI Fangfang;TANG Yangguo;FANG Zhishan;HE Baoxiang;DU Yulan(Xingjian College of Sciences and Liberal Arts,Guangxi University,Guangxi Province 530005 China;College of animal science and technology,Guangxi University,Guangxi Province 530005 China;Guangxi Animal Husbandry Research Institute,Guangxi Province 530005 China)
出处
《中国饲料》
北大核心
2022年第8期33-36,共4页
China Feed
基金
广西自然科学基金青年科学基金项目(2018GXNSFBA138015)
广西高校中青年教师科研基础能力提升项目(2018KY0787)。
关键词
奶水牛
瘦素
分娩前后
抗氧化
血浆水平
milk buffalo
leptin
around partum
antioxidant
plasma level
