摘要
灵芝是我国一类传统药用真菌的统称,具有极高的药用价值和经济价值。为了充分保护和利用该类野生药用真菌资源,本研究对采自广西壮族自治区南宁市的一株野生灵芝进行了菌株分离纯化培养,通过基于内转录间隔区(internal transcribed spacer,ITS)序列的分子生物学分析鉴定为四川灵芝Ganoderma sichuanense。以此为实验菌株,对该种的生物学特性和抗氧化活性进行了研究。探索了不同碳源、氮源、无机盐、pH、温度在固体培养条件下对野生四川灵芝菌丝生长速度的影响。对上述5因子进行单因子试验,从中筛选出4因子再进行正交试验。在试验范围内野生四川灵芝菌丝生长的最佳碳源为麦芽糖,氮源为牛肉膏,无机盐为KH_(2)PO_(4),最适pH7.0,温度30℃。通过正交试验进一步优化,得到最佳因子组合为麦芽糖30.0g/L、牛肉膏5.0g/L、KH_(2)PO_(4)1.0g/L、pH 6.0。针对液体培养过程中的多糖、三萜、多酚、抗坏血酸含量、超氧化物歧化酶活性及羟自由基清除能力变化进行了测定,发现该野生四川灵芝具有一定的抗氧化活性。
Ganoderma species,generally used as traditional medicine in China,have considerably high medicinal and economic values.A wild Ganoderma isolate collected from Nanning,Guangxi Zhuang Autonomous Region was purified and identified as Ganoderma sichuanense by molecular biology analysis based on ITS sequences,and its biological characteristics and antioxidant activities were studied.The effects of different carbon and nitrogen sources,inorganic salts,pH,and temperature on the mycelial growth rate under solid cultivation were investigated.Single-factor tests were conducted on the five factors,among which four factors were screened and subjected to an orthogonal test.The results showed that the optimal carbon and nitrogen sources,and inorganic salt for the mycelial growth of wild G.sichuanense were maltose and beef extract,and KH;PO;,respectively,under conditions of pH 7.0 at 30 ℃.Further optimization based on orthogonal test proved that the optimal composition of the culture medium was maltose 30.0 g/L,beef extract 5.0 g/L,KH_(2)PO_(4)1.0 g/L,and pH 6.0.The contents of polysaccharide,triterpenoid,polyphenol,and ascorbic acid,the variation of superoxide dismutase activity,and scavenging ability to hydroxyl radicals were determined during the liquid cultivation.It was found that the wild G.sichuanense owned antioxidant activity.
作者
钱坤
武冬梅
王豪
孙一翡
司静
崔宝凯
QIAN Kun;WU Dongmei;WANG Hao;SUN Yifei;SI Jing;CUI Baokai(Institute of Microbiology,School of Ecology and Nature Conservation,Beijing Forestry University,Beijing 100083,China;Biotechnology Research Institute,Xinjiang Academy of Agricultural and Reclamation Sciences,Xinjiang Production and Construction Group,Key Laboratory of Crop Germplasm Enhancement and Gene Resources Utilization,Shihezi 832000,Xinjiang,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2022年第4期601-617,共17页
Mycosystema
基金
新疆生产建设兵团重点领域科技攻关计划项目(2021AB004)
国家自然科学基金(U2003211)
北京林业大学杰出青年人才培育计划项目(2019JQ03016)。
关键词
野生药用真菌
灵芝
生物学特性
抗氧化活性
驯化
wild medicinal fungi
Ganoderma
biological characteristic
antioxidant activity
domestication
作者简介
Corresponding author:司静,E-mail:jingsi1788@126.com。ORCID:SI Jing(0000-0001-9229-0727);Corresponding author:崔宝凯,E-mail:cuibaokai@bjfu.edu.cn。ORCID:CUI Baokai(0000-0003-3059-9344);钱坤,ORCID:QIAN Kun(0000-0003-1564-8371)。
