摘要
目的探讨金线莲苷(Kin)对肿瘤坏死因子-α(TNF-α)诱导的人类风湿关节炎滑膜细胞的增殖及炎症因子、基质金属蛋白酶MMPs产生的影响。方法以TNF-α诱导人类风湿关节炎滑膜成纤维细胞,同时给予不同剂量的金线莲苷刺激,CCK-8法用来检测滑膜细胞的增殖,ELISA用来检测细胞上清液中炎症因子白介素-6(IL-6)、白介素-1β(IL-1β)、白介素-8(IL-8)的分泌,实时荧光定量PCR和Western Blot分别用来检测细胞中基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶-13(MMP-13)的m RNA水平和蛋白水平表达。结果5μg/mL的金线莲苷能显著抑制TNF-α诱导的滑膜细胞的增殖(P<0.05)和滑膜细胞上清液中炎症因子IL-6、IL-1β、IL-8的升高(P<0.05)以及细胞中基质金属蛋白酶MMP-3、MMP-13的异常表达(P<0.05),并极显著地抑制MMP1的异常表达(P<0.01);10μg/mL的金线莲苷能够极显著抑制TNF-α诱导的滑膜细胞的增殖(P<0.01)和细胞上清液中IL-6、IL-1β的升高(P<0.01)以及细胞中MMP-1、MMP-3、MMP-13的异常表达(P<0.05),并显著降低IL-8的升高。结论本研究揭示了金线莲苷对人类风湿关节炎滑膜细胞的作用机制,为金线莲苷治疗类风湿关节炎提供了一定参考。
Objective To investigate the effect of kinsenoside(Kin)on tumor necrosis factor-α(TNF-α)induced proliferation of human rheumatoid arthritis synoviocytes and the production of inflammatory factors and MMPs.Methods TNF-αwas used to induce human rheumatoid arthritis(RA)synovial fibroblasts,and different doses of Kin were given at the same time.CCK-8 method was used to detect the proliferation of synovial cells.ELISA was used to detect the secretion of inflammatory factors interleukin-6(IL-6),IL-1β,IL-8 in the supernatant of cells.RT-PCR and WB was used to detect the expression of Metalloproteinase-1(MMP-1),MMP-3,MMP-13 in cells.Results The results showed that 5μg/mL of Kin could significantly inhibit the proliferation of TNF-αinduced synovial cells(P<0.05),the increase of IL-6,IL-1β,IL-8 in the supernatant of synovial cells(P<0.05),and the abnormal expression of MMP-3 and MMP-13 in cells(P<0.05),extremely significantly inhibit and the abnormal expression of MMP-1(P<0.01);10μg/mL of Kin could extremely significantly inhibit TNF-αinduced proliferation of synovial cells(P<0.01),the increase of inflammatory factors IL-6 and IL-1βin supernatant(P<0.01),and the abnormal expression of MMP-1,MMP-3 and MMP-13 in synovial cells(P<0.05),and significantly inhibit the increase of IL-8.Conclusion This study reveales the mechanism of action of Kin on human rheumatoid arthritis synoviocytes,and provides some reference for the treatment of RA with Kin.
作者
许艳
崔海虹
朱静媛
XU Yan;CUI Hai-hong;ZHU Jing-yuan(Laboratory Department,Suzhou Hospital of Integrated Chinese and Western Medicine of J iangsu Province,Suzhou 215101)
出处
《湖北中医药大学学报》
2022年第2期38-42,共5页
Journal of Hubei University of Chinese Medicine
基金
2021年江苏省级重点研发计划(社会发展)专项资金项目(项目编号:BE2021673)
江苏省苏州市中西医结合医院院级课题(项目编号:YJ2021002)。
作者简介
通信作者:许艳(1981-),女,江苏省苏州市中西医结合医院主管检验师,研究方向:临床微生物检验,E-mail:616154933@qq.com。
