摘要
为明确薄壳山核桃Carya illinoinensis叶斑病病原,对不同地区的薄壳山核桃叶斑病发生情况进行调查,采集感病植株,采用组织分离法分离培养病原菌,并经过柯赫氏法则验证,结合形态学和系统发育树进行鉴定。结果表明:从田间两种感病症状的薄壳山核桃叶片上共分离出4种致病真菌,分别为共享镰孢Fusarium commune、Phaeosphaeria fuckelii、茶藨子葡萄座腔菌Botryosphaeria dothidea和灰葡萄孢Botrytis cinerea,回接到健康山核桃叶片后,发病率分别为90.9%,100%,75.0%,100%。该结果可为了解薄壳山核桃叶斑病的病原种类及防治提供参考。
To determine the pathogen of leaf spot of Carya illinoinensis,the occurrence of leaf spot of C.illinoinensis in different areas was investigated and infected plants were collected.Pathogens were isolated from damaged parts of leaves by tissue isolation and cultured,and pathogenicity was verified by Koch′s rule.Pathogens were identified by morphological characteristics and phylogenetic analysis.The results showed that four species of fungi were isolated from leaves with two symptoms of C.illinoinensis in fileds and finally identified as Fusarium commune,Phaeosphaeria fuckelii,Botryosphaeria dothidea and Botrytis cinerea.Meanwhile,four species of isolates were inoculated to healthy leaves of C.illinoinensis,which incidence rate were 90.9%,100%,75.0%and 100%,respectively.The results provided a reference for knowing pathogen and control of leaf spot of C.illinoinensis.
作者
吴天昊
翟敏
胡龙娇
宣继萍
巨云为
何姣
WU Tianhao;ZHAI Min;HU Longjiao;XUAN Jiping;JU Yunwei;HE Jiao(Forestry College,Nanjing Forestry University,Nanjing 210037,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China)
出处
《中国森林病虫》
2022年第2期16-23,共8页
Forest Pest and Disease
基金
江苏省林业科技创新与推广项目“薄壳山核桃高效栽培技术集成示范”(LYKJ[2020]04)。
关键词
薄壳山核桃
叶斑病
病原鉴定
系统发育树
Carya illinoinensis
leaf spot
pathogen identification
phylogenetic tree
作者简介
第一作者:吴天昊(1996-),男,江苏无锡人,在读硕士,研究方向为森林病理学,E-mail:171573888@qq.com;翟敏(1985-),男,江西宜春人,实验师,研究方向为植物栽培生理研究,E-mail:854315070@qq.com;通信作者:巨云为,副教授,研究方向为昆虫生物学和生态学,E-mail:656898633@qq.com。
