摘要
目的:比较生脉饮活性成分五味子醇乙在正常及心肌缺血模型大鼠的药物代谢动力学差异。方法:通过尾静脉注射垂体后叶素(2.00 U·kg^(-1))建立大鼠心肌缺血模型,在口服灌胃生脉饮后按时间点进行尾静脉取血并用UPLC检测,绘制血药浓度-时间曲线图,并利用PKSolver 2.0系统进行药代动力学拟合,比较五味子醇乙在心肌缺血和正常大鼠体内的药物代谢动力学参数。结果:五味子醇乙在大鼠体内的代谢过程符合非房室模型。心肌缺血模型大鼠的Cmax、AUC(0-inf)、MRT(0-inf)、Vz/F较正常大鼠有较为明显的增加而Cl/F减小。结论:心肌缺血模型大鼠相较于正常大鼠对生脉饮中五味子醇乙的吸收度更好且体内滞留时间延长。
Objective:To study the pharmacokinetics difference of the active ingredient,schisandrol B,in Shengmai-yin in normal and myocardial ischemia model rats.Method:Myocardial ischemia rat model was established by tail vein injection of posterior pituitary hormone(2.00 U·kg^(-1)).Blood sample was collected from the tail vein at time points after oral gavage of Shengmai-yin and detected by UPLC.Blood concentration-time profiles were plotted and pharmacokinetic fitting was carried out using PKSolver 2.0 system to compare pharmacokinetic parameter difference of schisandrol B in myocardial ischemia and normal rats.Result:The pharmacokinetic process of schisandrol B in rats conformed to the non-compartmental model.The Cmax,AUC(0-inf),MRT(0-inf)and Vz/F of the rats in myocardial ischemia rats showed a significant increase while Cl/F was decreased compared with normal rats.Conclusion:Myocardial ischemia model rats showes better absorption and prolonged retention time of schisandrol B in Shengmai-yin compared with normal rats.
作者
杨苛
周玮玲
刘心煜
张迎
樊瑜歆
胡慧玲
游宇
YANG Ke;ZHOU Wei-ling;LIU Xin-yu;ZHANG Ying;FAN Yu-xin;HU Hui-ling;YOU Yu(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu 611137,Sichuan)
出处
《中药与临床》
2022年第1期15-18,共4页
Pharmacy and Clinics of Chinese Materia Medica
基金
国家自然科学基金项目(No.81904088)。
作者简介
杨苛,男,硕士研究生在读,研究方向为中药新制剂、新剂型及生物药剂学,Email:953844373@qq.com;通讯作者:胡慧玲,教授,主要从事中药新制剂、新剂型、新工艺及生物药剂学研究,Email:huhuiling@cdutcm.edu.cn;通讯作者:游宇,博士,副教授,主要从事中药新制剂、新剂型及新工艺研究,Email:415416295@qq.com。
