摘要
研究旨在探索适合枸杞的潮霉素筛选剂量,为枸杞基因功能的研究、遗传转化提供参考。以宁夏枸杞无菌苗叶片为外植体,设置4个潮霉素浓度梯度,研究其对诱导愈伤组织和愈伤组织诱导分化芽的影响,以选出适合枸杞转化体筛选的抗生素剂量。结果表明,随着潮霉素浓度的升高,其对枸杞诱导愈伤组织和愈伤组织诱导芽分化的抑制作用加强。潮霉素浓度越高,枸杞叶片的出愈率越低,愈伤组织的褐化率越高,且叶片逐渐变黄、白化甚至死亡。将潮霉素作为抗性选择标记时,抗性浓度以2 mg/L为最佳。
This study aims to explore the screening dose of hygromycin suitable for Lycium barbarum,and lay a foundation for the study of gene function and genetic transformation of L.barbarum.In this study,the leaves of germless L.barbarum seedlings from Ningxia were used as explants,and four hygromycin concentration gradients were set to study the effect of hygromycin on the induction of callus and callus differentiation buds,to screen out the appropriate antibiotic dose for L.barbarum transformation body screening.The results showed that the increased concentration of hygromycin had a significant inhibitory effect on the induced callus and bud differentiation of L.barbarum.The higher the concentration of hygromycin,the lower the recovering rate of L.barbarum leaves was,and the browning rate of callus increased,and the leaves gradually became yellow,albino,and even died.Using hygromycin as a selective marker for resistance,the optimal concentration of hygromycin is 2 mg/L.
作者
樊云芳
刘露露
张晶
罗青
张波
曹有龙
FAN Yunfang;LIU Lulu;ZHANG Jing;LUO Qing;ZHANG Bo;CAO Youlong(Lycium Engineering Technology Research Center,Ningxia Academy of Agricultural and Forestry Sciences,Yinchuan 750002,Ningxia,China;Natural Resources Bureau of Longde County,Guyuan 756300,Ningxia,China)
出处
《农学学报》
2022年第4期57-60,共4页
Journal of Agriculture
基金
宁夏回族自治区全产业链创新示范项目“枸杞功能基因表达与调控分析”(QCYL-2018-04,YES-16-0403)
宁夏农林科学院对外合作项目“特异枸杞资源的呈色机制及调控研究”(DW-X-2018011)
国家自然科学基金项目“黑果枸杞花青素苷合成关键酶基因及上游调控序列的克隆与表达分析”(31260351)。
关键词
宁夏枸杞
潮霉素
抗性浓度
选择标记
遗传转化
Lycium barbarum
hygromycin
resistance concentration
selected marker
genetic transformation
作者简介
第一作者:樊云芳,女,1981年出生,副研究员,主要从事枸杞分子生物学研究。通信地址:750002宁夏银川黄河东路590号宁夏农林科学院枸杞工程技术研究所,Tel:0951-6886783,E-mail:majoriefyf@163.com。;通讯作者:曹有龙,男,1963年出生,研究员,主要从事枸杞研究。通信地址:750002宁夏银川黄河东路590号宁夏农林科学院枸杞工程技术研究所,Tel:0951-6886785,E-mail:youlongchk@163.com。
