摘要
目的:确定从艾叶中分离、纯化出的白色晶体为高纯度白坚木皮醇,并检测其纯度。对比分析9个产地艾叶中白坚木皮醇含量差异。方法:采用提取、结晶、重结晶的方法进行分离、纯化,通过核磁共振和高分辨-电喷雾离子源-质谱(HR-ESI-MS)数据鉴定其化学结构和相对分子质量,使用高效液相色谱-蒸发光散射检测器法检测其纯度。结果:从艾叶中分离、纯化出一种白色晶体,根据其HR-ESI-MS数据鉴定其化学结构为白坚木皮醇,纯度>99%。9个产地艾叶中的白坚木皮醇含量差异有统计学意义(P<0.05),这可能与产地和采收时间有关。结论:提供了一种工业化获取高纯度白坚木皮醇的方法,增加了艾叶的附加值。
Objective:The white crystals were separated from Artemisiae Argyi Folium and purified and the purity was checked.Finally,they were identified as high-purity L-quebrachitol.The content of L-quebrachitol in Artemisiae Argyi Folium from 9 producing areas was different and the reason was expounded.Methods:Through extraction,crystallization,and recrystallization,the white crystals were separated and purified.The chemical structure and molecular weight of the crystals were identified by nuclear magnetic resonance and high-resolution electrospray ionization mass spectrometry(HRESI-MS),and the purity was tested by high performance liquid chromatography-evaporative light scattering detector.Results:White crystals were separated and purified which were identified as L-quebrachitol,with purity>99%.The content of L-quebrachitol was significantly different among Artemisiae Argyi Foliums from different producing areas(P<0.05),which may be related to the producing area and harvesting time.Conclusion:This study provides a method for obtaining high-purity L-quebrachitol in industrial production,which increases the added value of Artemisiae Argyi Folium.
作者
陈阳
江丹
廖子蔚
王玉
孙代华
陈志元
CHEN Yang;JIANG Dan;LIAO Zi-wei;WANG Yu;SUN Dai-hua;CHEN Zhi-yuan(Jing Brand Chizhengtang Pharmaceutical Co.,Ltd.,Huangshi 435100,China;Hubei Traditional Chinese Medicine Formula Granule Engineering&Technology Research Center,Huangshi 435100,China)
出处
《中国现代中药》
CAS
2022年第4期611-615,共5页
Modern Chinese Medicine
基金
湖北省重点研发计划项目(2020ACA007-03)
湖北省技术创新专项(2019AEE001)。
关键词
艾叶
核磁共振
高纯度
白坚木皮醇
Artemisiae Argyi Folium
nuclear magnetic resonance
high purity
L-quebrachitol
作者简介
通信作者:陈志元,高级工程师,研究方向:中药现代化,Tel:0714-3188779,E-mail:5780137@qq.com。
