摘要
为了解吉林省牛瑟氏泰勒虫病感染情况,本试验根据牛瑟氏泰勒虫18S rRNA基因序列设计引物,应用聚合酶链式反应(PCR)方法对吉林省珲春、通化、安图、舒兰、龙井、辽源、白山共7个县市采集的247份血液样本进行了牛瑟氏泰勒虫病分子流行病学调查,并进行不同地区、不同饲养方式、不同性别及不同年龄之间的比较。结果显示,牛瑟氏泰勒虫总体阳性率为39.68%(98/247),其中通化阳性率最高为70.00%(14/20),龙井阳性率最低为20.00%(8/40),地区之间除安图和白山差异不显著(P>0.05)外,其他各地区之间均差异显著(P<0.05);散养牛阳性率64.44%(58/90)与规模化养殖牛阳性率25.48%(40/157)之间差异极显著(P<0.01);母牛阳性率38.46%(50/130)与公牛阳性率41.03%(48/117)之间差异不显著(P>0.05);1岁以内牛阳性率为56.67%(34/60),1~3岁牛阳性率为42.22%(38/90),3岁以上牛阳性率为26.80%(26/97),不同年龄间差异显著(P<0.05)。结果表明,吉林省牛瑟氏泰勒虫感染的情况普遍存在。本次调查为吉林省牛瑟氏泰勒虫病的有效防控提供了科学依据。
This study conducted a molecular epidemiological investigation of Theileria sergenti infection in Jilin province.A total of 247 blood samples were collected from cattle in Hunchun,Tonghua,Antu,Shulan,Longjing,Liaoyuan,Baishan of Jilin porvince and examined by PCR with primers from T.sergenti 18S rRNA gene sequence.The results showed that the overall positive rate of T.sergenti was 39.68%(98/247),with the highest positive rate of 70.00%(14/20)in Tonghua,and the lowest positive rate of 20.00%(8/40)in Longjing.No significant difference was noted between Antun and Baishan regions(P>0.05),but significant differences were observed in other regions(P<0.05).The positive rate of 64.44%(58/90)in free-range cattle and 25.48%(40/157)in large-scale cattle were significantly different(P<0.01).No significant difference was found between the positive rates of 38.46%(50/130)in cows and 41.03%(48/117)in bulls(P>0.05).The positive rates were 56.67%(34/60)in cattle under 1 year old,42.22%(38/90)in cattle from 1 to 3 years old,and 26.80%(26/97)in cattle over 3 years old,with significant differences among different ages(P<0.05).The results showed that the infection of T.sergenti was widespread in Jilin province.This investigation provides a scientific basis for the effective prevention and control of T.sergenti in cattle in Jilin province.
作者
闵鹏飞
宋建臣
许应天
王浩
杨冰祎
千慧燕
张爽
贾立军
MIN Peng-fei;SONG Jian-chen;XU Ying-tian;WANG Hao;YANG Bing-yi;QIAN Hui-yan;ZHANG Shuang;JIA Li-jun(Engineering Research Center of North-East Cold Region Beef Cattle Science&Technology Innovation,Ministry of Education,Yanbian University,Yanji 133002,China;Agricultural College,Yanbian University,Yanji 133002,China)
出处
《中国兽医杂志》
CAS
北大核心
2022年第1期5-9,共5页
Chinese Journal of Veterinary Medicine
基金
吉林省中青年科技创新领军人才及团队项目(20200301034RQ)
高等学校学科创新引智111计划资助(D20034)
延边大学科研创新团队项目(延大校发[2018]203号)。
作者简介
闵鹏飞(1998-),男,硕士生,主要从事动物流行病学研究,E-mail:2281404274@qq.com;通信作者:贾立军,E-mail:lijunjia1015@sohu.com。
