摘要
目的建立经典型热射病(CHS)大鼠模型,与病理学结果相对照,评价7.0T小动物MRI观察CHS大鼠脑损伤的价值。方法将20只雄性SD大鼠随机等分为高温组及常温组;以动物体温维持仪建立CHS大鼠模型(高温组),记录建模成功时间(发病时间),于建模前、后检测高温组大鼠血清肌酸激酶(CK)水平,观察其脑MRI变化,并与病理学所见进行对照。结果高温组大鼠CHS发病时间为(45.90±4.04)min;建模后血清CK水平高于建模前(P<0.01)。建模后采集2次梯度回波(GRE)-T2WI,高温组10只(10/10,100%)大鼠脑部均见散在点状低信号,病理学见散在微出血;建模后首次弥散加权成像(DWI)10只大鼠均未见明显异常,第2次DWI示6只(6/10,60.00%)皮层区信号较白质区增高,病理学见局灶性或弥漫性神经细胞肿大;建模后采集2次T1WI、T2WI及T2液体衰减反转恢复序列(FLAIR)均未见明显异常信号。结论利用7.0T小动物MR仪可无创、动态反映CHS大鼠模型早期脑组织损伤;GRE-T2WI及DWI可较准确地检出脑组织细胞毒性水肿及微出血。
Objective To explore the value of 7.0T small animal MR scanner for observation on brain injuries in rat models of classical heat stroke(CHS)compared with pathological findings.Methods Twenty male SD rats were randomly divided into high temperature group and normal temperature group(each n=10).CHS rat models(high temperature group)were established with animal temperature controller,and the successful time of modeling(onset time)was recorded.The level of serum creatine kinase(CK)before and after modeling in high temperature group were tested,and brain MRI changes before and after modeling were observed and compared with pathological changes of brain tissue.Results The onset time of CHS was(45.90±4.04)min,and the serum CK level after modeling was higher than that before modeling in high temperature group(P<0.01).Two times gradient echo(GRE)-T2WI after modeling showed scattered punctate low signals in the brain of 10 rats(10/10,100%)in high temperature group,while scattered microbleedings were observed with pathology.After modeling,the first time diffusion weighted imaging(DWI)showed no obvious abnormality in 10 rats,but the second time DWI found that the signals in cortical areas were higher than those in white matter areas in 6 rats(6/10,60.00%),while focal or diffused nerve cell swelling were noticed with pathology.No obvious abnormal signal was found on T1WI,T2WI nor two times of T2 fluid attenuated inversion recovery(FLAIR)after modeling.Conclusion 7.0T small animal MR scanner could be used to noninvasively and dynamically reflect early brain tissue injuries of CHS rat models,while cytotoxic edema and microbleeding of brain tissue could be accurately detected with GRE-T2WI and DWI.
作者
杭开兵
苏维维
黄静
鲍光进
刘文豪
李树平
HANG Kaibing;SU Weiwei;HUANG Jing;BAO Guangjin;LIU Wenhao;LI Shuping(Department of Diagnostic Radiology,Naval Characteristic Medical Center of People's Liberation Army,Shanghai 200052,China;Department of Anatomy,Histology&Embryology,Medical College,Fudan University,Shanghai 200032,China)
出处
《中国医学影像技术》
CSCD
北大核心
2022年第4期481-485,共5页
Chinese Journal of Medical Imaging Technology
基金
海军特色医学中心院内基金(18M4701)。
关键词
中暑
大鼠
磁共振成像
heat stroke
rats
magnetic resonance imaging
作者简介
第一作者:杭开兵(1989-),男,江苏盐城人,本科,主治医师。研究方向:神经系统影像诊断。E-mail:1169020313@qq.com;通信作者:李树平,中国人民解放军海军特色医学中心放射诊断科,200052。E-mail:20739293@qq.com。
