摘要
结核性葡萄膜炎因难以获得病原学诊断依据,至今仍很难明确诊断。聚合酶链式反应(polymerase chain reaction,PCR)技术可扩增痕量级核酸的特点对少菌性感染的结核性葡萄膜炎而言是一大优势。在临床实际应用中,PCR技术的类别、靶基因序列的选用、眼内液样本类型及患者的选择等在一定程度上可能影响PCR检测的敏感度。对结核性葡萄膜炎致病机理及抗结核药物治疗反应等的深入剖析有助于临床医生从不同角度更好地分析PCR检测结果及治疗结局。
Nowadays,the diagnosis of tuberculous uveitis is still difficult to confirm for whose pathogen is nearly unavailable.As a popular molecular detection method that could amplify the nucleic acid of specific pathogens with trace samples,polymerase chain reaction(PCR)is suitable for the diagnosis of tuberculous uveitis.In clinical practice,the types of PCR technology,intraocular fluid sample and the selection of the target gene sequence and patients,may affect the sensitivity of the PCR detection to a certain extent.Better understanding of the pathogenic mechanism and the anti-tuberculosis response in tuberculous uveitis will do a great help for clinicians to analyze the PCR detection results and treatment outcome from different perspectives.
作者
许惠容
陈放
Xu Huirong;Chen Fang(Department of Ophthalmology,Northern Jiangsu People’s Hospital,Yangzhou University,Yangzhou 225001,China;Graduate School,Dalian Medical University,Dalian 116044,China)
出处
《国际眼科纵览》
2022年第1期66-70,共5页
International Review of Ophthalmology
基金
扬州科技发展计划项目(YZ2020112)。
作者简介
通信作者:陈放,Email:cfyzsbyy@163.com。
