摘要
为提高甘草种子及幼苗的耐盐碱性,本实验采用培养皿纸上发芽法,以甘草种子(Glycyrrhiza uralensis Fisch.)为试验材料,研究γ-氨基丁酸(GABA)对盐、碱和盐碱胁迫下甘草种子萌发特性和抗氧化酶活性的影响。结果表明,0.1 mmol·L^(-1)的GABA显著提高盐胁迫下种子的活力指数,促进胚轴长度;0.2 mmol·L^(-1)的GABA可有效提高碱胁迫下种子的发芽势,0.5 mmol·L^(-1)的GABA提高胚根长;盐碱胁迫下,胚根和鲜重在0.3 mmol·L^(-1)的GABA处理下显著增加。GABA也可显著提高3种胁迫下的抗氧化酶活性,其中,0.05 mmol·L^(-1)的GABA可显著提高3种胁迫下的过氧化物酶(POD)活性。0.2 mmol·L^(-1)的GABA提高盐胁迫下超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性。0.3 mmol·L^(-1)的GABA提高碱和盐碱胁迫下的CAT活性,0.5 mmol·L^(-1)的GABA可提高其SOD活性。综上,GABA可通过提高种子发芽势、活力指数和抗氧化酶活性来缓解盐碱胁迫对甘草种子萌发的影响,提高甘草幼苗的耐盐碱能力。
In order to improve the saline-alkaline tolerance of Glycyrrhiza uralensis seeds,we studied the effects of γ-aminobutyric acid(GABA)on germination characteristics and antioxidant enzyme activity of G. uralensis under saline,alkaline,and saline-alkaline stresses using the petri dish germination method. The results showed that 0.1 mmol·L^(-1)GABA significantly increased the vitality index of seeds under saline stress,and increased,hypocotyl length. 0.2 mmol·L^(-1)GABA effectively increased germination potential,0.5 mmol·L^(-1)GABA increases radicle length under alkaline stress. Under saline-alkaline stress,0.3 mmol·L^(-1)GABA significantly increased radicle and fresh weight. GABA also significantly increased the activity of antioxidant enzymes under the three stresses,and 0.05 mmol·L^(-1)GABA significantly increased the activity of peroxidase(POD)under the three stresses. 0.2 mmol·L^(-1)GABA increased the activities of superoxide dismutase(SOD)and catalase(CAT)under saline stress. 0.3 mmol·L^(-1)GABA increased CAT activity under alkaline and saline-alkaline stresses. 0.5 mmol·L^(-1)GABA increased SOD activity. In summary,these results suggested that GABA could alleviate the effects of saline-alkaline stress on seed germination of G. uralensis,and increase saline-alkaline tolerance in G. uralensis seedling via improving seed germination potential,vigor index and antioxidant enzyme activity.
作者
葛甜甜
王楠
高静
关思静
徐蓉蓉
张亚丽
谢丰璞
李瑞
严加坤
颜永刚
张岗
Ge Tiantian;Wang Nan;Gao Jing;Guan Sijing;Xu Rongrong;Zhang Yali;Xie Fengpu;Li Rui;Yan Jiakun;Yan Yonggang;Zhang Gang(College of Pharmacy,Shaanxi University of Chinese Medicine,Xi’an 712046,China;State Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Co-con-struction Collaborative Innovation Center for Chinese Medicine Resources Industrialization by Shaanxi&Education Ministry,Shaanxi University of Chinese Medicine,Xianyang 712083,China;College of Life Sciences,Yulin College,Yulin 719000,China)
出处
《中国野生植物资源》
CSCD
2022年第3期1-7,共7页
Chinese Wild Plant Resources
基金
国家自然科学基金项目(31600320)
陕西中医药大学“秦药”品质评价及资源开发学科创新团队项目(2019-QN01)
陕西省重点研发计划(2019TSLNY03-1)
陕西省社会发展科技攻关项目(2018SF-338)。
关键词
盐碱胁迫
Γ-氨基丁酸
种子萌发
抗氧化酶活性
Saline-alkaline stress
γ-Aminobutyric acid
Seed germination
Antioxidant enzyme activity
作者简介
葛甜甜(1994-),女,陕西子洲人,硕士研究生,研究方向为中药资源的开发与利用研究。E-mail:gt2020053@163.com;通讯作者:高静(1986-),女,山东淄博人,副教授,博士,研究方向为中药资源的开发与利用研究。E-mail:gaojing@sntcm.edu.cn。
