摘要
目的:建立中药材中7种真菌毒素的液相色谱-四极杆串联离子阱复合质谱法测定方法。方法:样品经乙腈-甲酸-水(75:1:24)提取,MLJ-1多重基质吸附型固相萃取柱净化,Shim-pack GIST C18色谱柱分离,流动相为乙腈和0.01mol/L乙酸铵水溶液(含0.1%甲酸)梯度洗脱,电喷雾正离子模式电离,质谱扫描模式为多反应监测-信息关联扫描-增强子离子扫描(MRM-IDA-EPI),外标法定量。结果:经方法学验证,方法的回收率、精密度均符合要求。结论:本方法应用相色谱-四极杆串联离子阱复合质谱特有的MRM-IDA-EPI扫描模式,可实现中药材中7种真菌毒素准确的定性、定量分析及可疑峰筛查、确证。
Objective:A method for the determination of seven mycotoxins in traditional Chinese medicine by liquid chroma⁃tography quadrupole tandem ion trap composite mass spectrometry was established.Method:Samples were extracted with acetonitrile and formic acid and water(75:1:1:24),purified by MLJ-1 purification of solid phase extraction column with multiple matrix adsorption,The mobile phases were acetonitrile and 0.01 mol/L ammonium acetate with 0.1%formic acid in gradient elution,ionized by electrospray positive ion mode,detected by MRM-IDA-EPI scanning mode and quantified by external standard method.Results:The recovery and precision of the method meet the requirements.Conclusion:The MRM-IDA-EPI scanning mode of liquid chromatography quadrupole tandem ion trap composite mass spectrometry was used to realize the accurate qualitative and quantitative analysis of seven mycotoxins in traditional Chinese medicine and the screening and confirmation of suspicious peaks.
作者
刘瑜
房建美
胡姝
姜莉
李志远
赵余庆
Liu Yu;Fang Jing-mei;Hu Shu;Jiang Li;Li Zhi-yuan;Zhao Yu-Qing(Shenyang Customs Technology Center,Shenyang,Liaoning 110016,China;Shanghai AB Sciex Analytical Instrument Trading Co.,Ltd.,Shanghai 100015,China;Shenyang Pharmaceutical University,Shenyang,Guangdong 110016,China)
出处
《福建分析测试》
CAS
2022年第2期1-4,共4页
Fujian Analysis & Testing
基金
辽宁省自然科学基金指导计划项目(2019-ZD-0867)。
关键词
中药材
真菌毒素
超高效液相色谱-四极杆串联离子阱复合质谱
traditional Chinese medicine
mycotoxin
liquid chromatography quadrupole tandem ion trap composite mass spectrometry
作者简介
刘瑜(1982-),女,硕士,高级工程师,从事食品理化检验工作。E-mail:liuyu-0117@163.com。
