摘要
目的:探讨玛卡多糖抗非小细胞肺癌作用及其机制。方法:采用一定浓度的玛卡多糖处理非小细胞肺癌A549细胞,CCK-8法、划痕实验和Transwell小室检测玛卡多糖对A549细胞增殖、迁移和侵袭的影响;流式细胞术检测玛卡多糖对A549细胞周期和凋亡的影响;实时荧光定量PCR和Western Blot法检测细胞周期和凋亡相关因子的表达。结果:玛卡多糖能显著抑制A549细胞的增殖、迁移和侵袭;细胞周期被阻滞在G_(0)/G_(1)期和G_(2)/M期;实时荧光定量PCR结果显示,CDK-1和Cyclin B1的表达显著降低,Caspase-3、Caspase-8、Caspase-9的表达显著升高;Western Blot结果发现,周期相关蛋白CDK-1、Cyclin B1、CDK-4、CDK-6、Cyclin D1的表达显著降低,凋亡相关蛋白Caspase-3、Caspase-8、Caspase-9、Bax的表达显著升高,Bcl-2的表达显著降低。结论:玛卡多糖能够抑制非小细胞肺癌A549细胞的增殖并促进细胞凋亡。
OBJECTIVE To investigate the effects and mechanism of Maca polysaccharide on non-small cell lung cancer.METHODS Non-small cell lung cancer A549 cells were treated with Maca polysaccharide at a certain concentration, and the effects of Maca polysaccharide on the proliferation, migration and invasion of A549 cells were observed by CCK-8 scratch assay and transwell chamber. Flow cytometry was used to detect the effects of Maca polysaccharide on the cell cycle and apoptosis of A549 cells. The expression of cell cycle and apoptosis related factors were measured by real-time quantitative PCR and Western Blot.RESULTS Maca polysaccharides could significantly inhibit the proliferation, migration and invasion of A549 cells. The cell cycle was arrested in G_(0)/G_(1)and G_(2)/M phases. Real-time quantitative PCR showed that the expression levels of CDK-1 and Cyclin B1 were significantly decreased, while the expression levels of Caspase-3, Caspase-8 and Caspase-9 were significantly increased. Western blot indicated that the expression of cyclin-related protein CDK-1, Cyclin B1, CDK-4, CDK-6 and Cyclin D1 were significantly down-regulated, while the expression of apoptosis-related proteins Caspase-3, Caspase-8, Caspase-9 and Bax were significantly up-regulated and Bcl-2 was significantly reduced.CONCLUSION Maca polysaccharides can inhibit the proliferation and promote the apoptosis of non-small cell lung cancer A549 cells.
作者
程婷婷
刘涵
邓云青
李倩倩
黄丽金
李自霖
陈贵元
CHENG Ting-ting;LIU Han;DENG Yun-qing;LI Qian-qian;HUANG Li-jin;LI Zi-lin;CHEN Gui-yuan(College of Basic Medicine,Dali University,Yunnan Dali 671000,China;Clinical Medical College of Dali University,Yunnan Dali 671000,China;Provincial Key Laboratory of Entomology Biopharmaceutical R&D,Yunnan Dali 671000,China)
出处
《中国医院药学杂志》
CAS
北大核心
2022年第5期491-494,518,共5页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金项目(编号:31860252)
云南省自然科学基金高校联合面上项目(编号:2017FH001-084)
大理大学博士科研启动费项目(编号:KYBS201401)。
关键词
玛卡多糖
非小细胞肺癌
细胞增殖
细胞周期
凋亡
Maca polysaccharide
non-small cell lung cancer
cell proliferation
cell cycle
apoptosis
作者简介
程婷婷,女,硕士研究生在读,研究方向:天然产物的分离纯化与生物学活性;刘涵,女,硕士研究生在读,研究方向:天然产物的分离纯化与生物学活性;通信作者:陈贵元,男,博士,副教授,硕士生导师,研究方向:天然产物的分离提取纯化及生物学活性。
