摘要
目的探讨消皮素(gasdermin,GSDM)基因家族在原发性肝癌中的表达及其临床意义。方法通过基因表达谱数据动态分析(GEPIA2)数据库分析GSDM基因家族在原发性肝癌与正常组织中的表达水平差异,并进行生存分析探索其与预后的关系;再利用GEPIA2数据库探索GSDM基因家族与原发性肝癌患者TNM分期的关系;通过GeneMANIA数据库预测可能与GSDM基因家族相互作用的基因,并利用Metascape网站对其进行功能富集分析;最后,利用TIMER数据库探索GSDM基因家族的表达与原发性肝癌肿瘤微环境当中免疫细胞浸润的关系。结果与正常肝组织比较,GSDM基因家族中的GSDMA、GSDMC、GSDMD以及GSDME在原发性肝癌组织中呈高表达(P<0.050),GSDMB和DFNB59呈低表达(P<0.050);单因素Cox比例风险回归模型结果表明,GSDMD、GSDME以及DFNB59的差异表达与患者的总生存相关(P<0.050),且多因素Cox比例风险回归模型结果表明GSDME可作为肝癌患者预后的预测因子(P<0.050)。随着肝癌患者TNM分期的增加,GSDMA和GSDMC的表达量也逐渐增加(P<0.050)。进一步的富集分析表明GSDM基因家族参与细胞焦亡及多种与免疫相关的生物学过程。结论GSDM基因在原发性肝癌当中差异表达,参与了免疫相关的生物学过程,并且其表达和临床病理分期以及患者预后相关。
Objective To investigate the expression of gasdermin(GSDM)gene family in primary liver cancer and its clinical significance.Methods The Gene Expression Profile Data Dynamic Analysis(GEPIA2)database was used to analyze the expression levels of GSDM gene family in primary liver cancer and normal tissues,and survival analysis was performed to explore its relationship with prognosis;GEPIA2 database was used to explore the relationship between GSDM gene family and TNM staging of patients with primary liver cancer.We used GeneMANIA database to predict genes that may interact with GSDM gene family,and used Metascape website for functional enrichment analysis.Finally,we used TIMER database to explore the relationship of expression of GSDM gene family and immune cell infiltration in the tumor microenvironment of primary liver cancer.Results Compared with normal liver tissues,GSDMA,GSDMC,GSDMD,and GSDME were highly expressed in primary liver cancer(P<0.050),and GSDMB and DFNB59 were low expressed(P<0.050);results of univariate Cox proportional hazard regression model showed that the differential expressions of GSDMD,GSDME,and DFNB59 were related to the overall survival of patients(P<0.050),and the results of the multivariate Cox proportional hazard regression model showed that GSDME could be used as an independent predictor of the prognosis of liver cancer patients(P<0.050).With the increase of TNM staging in patients with liver cancer,the expressions of GSDMA and GSDMC also gradually increased(P<0.050).Further enrichment analysis showed that the GSDM gene family was involved in pyrolysis and various immune-related biological processes.Conclusion The GSDM gene is differentially expressed in primary liver cancer,participates in immune-related biological processes,and its expression is related to clinicopathological staging and patients’prognosis.
作者
郭影
郭秋影
GUO Ying;GUO Qiuying(Department of Hepatology,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,P.R.China;The Second Department of Orthopedics,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,P.R.China)
出处
《中国普外基础与临床杂志》
CAS
2022年第3期328-336,共9页
Chinese Journal of Bases and Clinics In General Surgery
关键词
原发性肝癌
消皮素基因
数据分析
预后
Primary liver cancer
gasdermin gene
data analysis
prognosis
作者简介
通信作者:郭秋影,Email:516512664@qq.com。
