摘要
[目的]筛选红豆杉NAC转录因子家族中影响根系形成的关键基因,探索红豆杉根系生长发育分子机理。[方法]利用曼地亚红豆杉全长转录组数据,通过生物信息学方法鉴定NAC转录因子,并对筛选出的NAC转录因子进行蛋白结构及基因组织表达谱等分析。[结果]共鉴定出44个NAC转录因子,其在N端均具有典型的保守NAC结构域,分别聚类到拟南芥的7个亚家族中,蛋白三级结构较为相似,且成员大多含有5个保守亚结构域。组织表达谱显示TmNAC15、16、18、21、22、29、39、40、41和44在根中表达水平高于茎和叶。[结论]从曼地亚红豆杉中共鉴定出44个NAC转录因子,其结构较为保守,且聚类为7个亚家族,其中成员TmNAC21、22、39、40和44极有可能参与红豆杉根系生长发育。
[Objective]To obtain the key NAC genes involving in regulating the root growth and development of yew trees.[Method]Basing on the full-length transcriptome data of Taxus×media,bioinformatics methods were used to identify NAC transcription factors.The protein structure and gene tissue expression profile of the selected NAC genes were also analyzed.[Result]A total of 44 NAC transcription factors were identified,which have typical conserved NAC domains at the N-terminal,and most of the members contain 5 conserved structural functional subdomains.Phylogenetic tree analysis showed that 44 T.×media NAC(TmNAC)proteins were clustered into 7 subfamilies of Arabidopsis.Sequence structure analysis indicated that the tertiary structures of the TmNAC proteins were similar,but the structures of the representative member of different subfamily also showed obvious differences.The expression results showed that TmNAC15,16,18,21,22,29,39,40,41,and 44 had higher expression level in roots than stems and needles.[Conclusion]In this study,44 TmNAC transcription factors were identified from T.×media and they were clustered into 7 subfamilies.Among them,TmNAC21,22,39,40,and 44 may be involved in the root growth and development progresses of yew trees.
作者
张恺恺
杨立莹
丰美静
张林凤
陈段芬
邱德有
杨艳芳
ZHANG Kai-kai;YANG Li-ying;FENG Mei-jing;ZHANG Lin-feng;CHEN Duan-fen;QIU De-you;YANG Yan-fang(Research Institute of Forestry,Chinese Academy of Forestry,State Key Laboratory of Forest Genetics and Breeding,Key Laboratory of Forest Cultivation,National Forestry and Grassland Administration,Beijing 100091,China;College of Horticulture,Hebei Agricultural University,Baoding 071001,Hebei,China;China Flower Association,Beijing 100020,China)
出处
《林业科学研究》
CSCD
北大核心
2022年第2期97-103,共7页
Forest Research
基金
“十三五”国家重点研发计划子课题“红豆杉高效培育技术研究”(2017YFD060070605)
国家林业和草原局科技发展中心项目(KJZXSA202037)
国家自然科学基金项目(31570675)项目资助。
关键词
红豆杉
NAC基因
基因鉴定
表达分析
Taxus spp.
NAC gene
gene identification
expression analysis
作者简介
通讯作者:杨艳芳,博士,副研究员.主要研究方向:林木次生代谢与调控.电话:010-62889638.E-mail:echoyyf@caf.ac.cn。
