摘要
分别使用石油醚、乙酸乙酯、70%乙醇等3种不同极性溶剂萃取紫茎泽兰叶的有效成分,通过DPPH法、ABTS法、FRAP法评价萃取物的体外抗氧化活性。结果表明,紫茎泽兰3种萃取物均有一定的抗氧化活性,其中70%乙醇萃取物对DPPH自由基和ABTS自由基的清除能力及总抗氧化能力最强,IC_(50)值分别为0.074 g·L^(-1)、0.024 g·L^(-1),FRAP值为3.47±0.01。3种评价结果显示紫茎泽兰萃取物具有良好的抗氧化活性,为进一步利用紫茎泽兰开发天然抗氧化剂奠定了基础。
We extract the active components from Eupatorium adenophorum Spreng leaves by petroleum ether,ethyl acetate,and 70%ethanol,and evaluated the antioxidant activities in vitro of the extracts by DPPH method,ABTS method,and FRAP method.The results show that all the three extracts have certain antioxidant activities,and the 70%ethanol extract has the strongest scavenging ability on DPPH free radicals and ABTS free radicals and total antioxidant ability,with IC_(50) value of 0.074 g·L^(-1) and 0.024 g·L^(-1),respectively,and FRAP value of 3.47±0.01.The three evaluation results show that the extracts of Eupatorium adenophorum Spreng have antioxidant activity in vitro.The study lays a foundation for further exploitation of natural antioxidants using Eupatorium adenophorum Spreng.
作者
张朝志
袁彩莲
程依婷
佘容
肖文
ZHANG Chaozhi;YUAN Cailian;CHENG Yiting;SHE Rong;XIAO Wen(Institute of Natural Antioxidants and Oxidant Inflammation,Dali University,Dali 671003,China;Institute of Eastern-Himalaya Biodiversity Research,Dali University,Dali 671003,China;Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China,Dali 671003,China;The Provincial Innovation Team of Biodiversity Conservation and Utility of the Three Parallel Rivers Region,Dali University,Dali 671003,China)
出处
《化学与生物工程》
CAS
2022年第3期35-39,55,共6页
Chemistry & Bioengineering
基金
第二次青藏高原综合科学考察研究资助项目(2019QZKK0402)
国家自然科学基金地区项目(31860164)。
关键词
入侵物种
紫茎泽兰
抗氧化活性
自由基清除率
总抗氧化能力
invasive species
Eupatorium adenophorum Spreng
antioxidant activity
free radical scavenging rate
total antioxidant ability
作者简介
张朝志(2001-),女,云南昆明人,研究方向:天然产物开发与利用,E-mail:2981273494@qq.com;通讯作者:佘容(1982-),高级工程师,E-mail:sher@eastern-himalaya.cn。
