摘要
为了了解硒化甘草多糖(SeGUP)对雏鸡抗炎活性的影响,试验采用脂多糖(LPS)建立雏鸡体内炎症模型,选取300只1日龄京红1号雏鸡,随机分为5组,分别为空白对照组、LPS组、LPS+黄芪多糖(APS)组、LPS+甘草多糖(GUP)组、LPS+硒化甘草多糖(SeGUP)组,除空白对照组外,各组试验鸡于8,10,12日龄腹腔注射LPS后,LPS+APS组、LPS+GUP组、LPS+SeGUP组分别肌肉注射给予浓度为1 mg/mL的APS、GUP、SeGUP,0.5 mL/只,连续7 d,分别于试验鸡18,25,32,39,46日龄每组选取10只,采用碳粒廓清法检测雏鸡巨噬细胞吞噬指数,用试剂盒检测雏鸡血清中肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、白细胞介素2(IL-2)、白细胞介素6(IL-6)、一氧化氮(NO)和诱导型NO合酶(iNOS)含量。结果表明:与空白对照组比较,LPS组18,25,32,39,46日龄雏鸡巨噬细胞吞噬指数和血清中IL-1β、IL-2、IL-6、TNF-α、NO、iNOS含量均显著升高(P<0.05);与LPS组比较,LPS+SeGUP组18,25,32,39,46日龄雏鸡巨噬细胞吞噬指数和血清中IL-1β、IL-2、IL-6、TNF-α、NO、iNOS含量均显著降低(P<0.05)。说明SeGUP能缓解雏鸡因LPS刺激引起的炎性反应,从而发挥抗炎作用。
In order to understand the effect of selenizing Glycyrrhiza uralensis polysaccharide(SeGUP) on anti-inflammatory activity in chicks, the experiment used lipopolysaccharide(LPS) to establish an inflammation model in chicks. Three hundred 1-day-old Jinghong No.1 chicks were selected and randomly divided into five groups, namely the blank control group, the LPS group and the LPS + astragalus polysaccharide(APS) group, the LPS + Glycyrrhiza uralensis polysaccharide(GUP) group, the LPS + selenizing Glycyrrhiza uralensis polysaccharide(SeGUP) group. Except blank control group, after intraperitoneal injection of LPS in experimental chickens at 8, 10, and 12 days old, the LPS+APS group, the LPS+GUP group, and the LPS+SeGUP group were given APS, GUP, and SeGUP at a concentration of 1 mg/mL by instramuscular injection, respectively, 0.5 mL per chick for seven days. Ten chickens were chosen from each group at 18, 25, 32, 39, 46 days old. The phagocytic index of chicken macrophages was detected by carbon clearance method;the kits were used to detect the contents of tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β), interleukin 2(IL-2), interleukin 6(IL-6), and nitric oxide(NO) in the serum of chicks and inducible NO synthase(iNOS). The results showed that compared with the blank control group, 18, 25, 32, 39, 46 day-old chick macrophage phagocytic index and serum IL-1β, IL-2, IL-6, TNF-α, NO, iNOS levels were significantly increased in the LPS group(P<0.05);compared with the LPS group, 18, 25, 32, 39, 46 day-old chick macrophage phagocytic index and serum IL-1β, IL-2, IL-6, TNF-α, NO, iNOS levels were significantly decreased in the LPS+SeGUP group(P<0.05). The results suggested that SeGUP could alleviate the inflammatory response caused by LPS stimulation in chicks and played an anti-inflammatory effect.
作者
丁艳
艾子凯
王虹
武梦丽
孙国结
连科迅
谷新利
DING Yan;AI Zikai;WANG Hong;WU Mengli;SUN Guojie;LIAN Kexun;GU Xinli(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2022年第4期115-120,共6页
Heilongjiang Animal Science And veterinary Medicine
基金
石河子大学高层次人才科研启动项目(RCZK201903)。
关键词
硒化甘草多糖
京红1号
雏鸡
脂多糖
炎症模型
抗炎
selenizing Glycyrrhiza uralensis polysaccharide
Jinghong No.1
chick
lipopolysaccharide
inflammatory model
anti-inflammatory
作者简介
丁艳(1997—),女,硕士研究生,研究方向为兽药创制与动物疾病诊断监控技术,3300633509@tjq.com;通信作者:连科迅(1986—),男,讲师,博士,研究方向为新型生物制剂开发与应用,liankexun@shzu.edu.cn;谷新利(1963-),男,教授,博士,博士生导师,研究方向为中兽药开发与应用,xlgu@shzu.edu.cn.
