摘要
TCP转录因子是参与植物生长发育的特异性家族蛋白,但是棉花TCP转录因子家族中的大多数成员功能尚不清楚。为了定位GbTCP5转录因子调控的靶基因,本研究通过Plant CARE在线网站分析棉花纤维发育基因GbGL3上游3 000 bp启动子中的顺式作用元件,构建含有TCP顺式作用元件的载体PHISII-GbGL3和酵母表达载体p GADT7-GbTCP5,通过酵母单杂交分析GbTCP5与GbGL3启动子中TCP顺式作用元件的结合特性。结果表明:GbGL3上游3 000 bp启动子中包含TCP顺式用作元件和功能各异的顺式作用元件,酵母单杂交实验结果表明GbTCP5能够与GbGL3启动子中含有的TCP顺式用作元件结合。本研究为进一步了解GbTCP5转录因子的功能提供依据。
TCP transcription factors are specific family proteins involved in plant growth and development. However, the function of most members of the family of transcription factors TCP cotton remains unclear. This study analyzed cis-acting elements in 3 000 bp promoter upstream of cotton fiber development gene GbGL3 online website, and the cis-acting element vector and yeast expression vector were constructed respectively. The binding characteristics of cis-acting elements in GbTCP5 and GbGL3 promoter were analyzed by yeast one-hybrid method.The results showed that in addition to TCP cis-acting elements, the upstream 3 000 bp promoter in GbGL3 was used as a component. The PCR and sequencing of bacterial solution confirmed the pGADT7-GbTCP5 and PHISII-GbGL3 recombinant plasmids and the combination of cis-acting elements in yeast. This study provides a basis for further understanding the target genes of GbTCP5 transcription factors.
作者
王怡
于月华
万会娜
蔡永生
倪志勇
Wang Yi;Yu Yuehua;Wan Huina;Cai Yongsheng;Ni Zhiyong(College of Agronomy,Xinjiang Agricultural University,Urumqi,830052)
出处
《分子植物育种》
CAS
北大核心
2022年第3期847-851,共5页
Molecular Plant Breeding
基金
自治区天山创新团队计划项目(2020D14002)
国家自然科学基金项目(U1703115
U1903203)
自治区天山青年计划项目(2018Q002
2018Q018)
棉花生物学国家重点实验室开放课题基金项目(CB2020A23)
新疆农业大学作物学重点学科发展基金项目(XNCDKY2019005)和新疆农业大学研究生科研创新项目(XJAUGRI2019-001)共同资助。
作者简介
通信作者:倪志勇,nizhiyong@126.com。
