摘要
目的:建立虾青素软胶囊中虾青素含量的测定方法。方法:采用YMC Carotenoid色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-叔丁基甲基醚^(-1)%磷酸溶液,进行梯度洗脱;紫外检测器检测,检测波长为474 nm;流速为1.0 mL·min^(-1);进样量为20μL。结果:虾青素为0.01~0.03 mg·mL^(-1)时线性关系良好,相关系数大于0.999,虾青素的回收率为99.2%~99.4%,RSD为1.5%。结论:该方法具有良好的专属性、精密度、线性和准确度,适用于虾青素软胶囊中虾青素的质量控制。
Objective:To establish a method for determination of astaxanthin esters in astaxanthin esters soft capsules.Method:YMC Carotenoid analytical column(250 mm×4.6 mm,5μm)was adopted with the mobile phase of acetonitriletert-butyl methyl ether^(-1)%phosphoric acid solution(gradient elution)at a flow rate of 1.0 mL·min^(-1).The detection wavelength was at 474 nm and the injection volume was 20μL.Result:The method had good linear relationship within the range 0.01~0.03 mg·mL^(-1)for astaxanthin,with the correlation coefficients were greater than 0.999,astaxanthin recoveries were between 99.2%and 99.4%,with RSD of 1.5%.Conclusion:The method has good specificity,precision,linearity,accuracy and can be used for quality control of astaxanthin esters in astaxanthin esters soft capsules.
作者
张建喜
周云婷
李俊行
刘剑萍
ZHANG Jianxi;ZHOU Yunting;LI Junhang;LIU Jianping(Zhejiang Novus Pharmaceuticals Co.,Ltd.,Shaoxing 312000,China;Xinchang Inspection and Testing Center for Food and Drug Control,Xinchang 312500,China;Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China;Xinchang Pharmaceutical Factory,Zhejiang Medicine Co.,Ltd.,Xinchang 312500,China)
出处
《现代食品》
2022年第4期185-188,193,共5页
Modern Food
关键词
虾青素软胶囊
虾青素
HPLC
酶解法
astaxanthin esters soft capsules
astaxanthin
HPLC
enzymatic hydrolysis
作者简介
张建喜(1977—),男,本科,工程师,研究方向为食品药品检测。
