摘要
目的研究STAT3、NCK1、VEGF在甲状腺乳头状癌中的表达情况及临床意义。方法收集2019年9月-2020年12月在唐山市工人医院头颈外科经病理诊断明确的甲状腺乳头状癌组织63例,甲状腺腺瘤组织32例,正常甲状腺组织20例。使用免疫组织化学法检测STAT3、NCK1、VEGF的表达水平,分析不同甲状腺组织中STAT3、NCK1、VEGF蛋白表达情况,以及甲状腺乳头状癌中STAT3、NCK1、VEGF蛋白与临床病理的关系。结果甲状腺乳头状癌中STAT3、NCK1、VEGF阳性表达率高于甲状腺腺瘤和正常甲状腺组织,差异有统计学意义(P<0.05);而甲状腺腺瘤和正常甲状腺组织中STAT3、NCK1、VEGF阳性表达率比较,差异无统计学意义(P>0.05);STAT3阳性表达与肿瘤直径、是否伴有颈部淋巴结转移相关(P<0.05),而与性别、年龄、病灶数、是否侵犯甲状腺外被膜、是否合并桥本甲状腺炎无关(P>0.05);NCK1阳性表达与是否伴有颈部淋巴结转移相关(P<0.05),而与性别、年龄、肿瘤直径、病灶数、是否侵犯甲状腺外被膜、是否合并桥本甲状腺炎无关(P>0.05);VEGF阳性表达与肿瘤直径、是否侵犯甲状腺外被膜和是否伴有颈部淋巴结转移相关(P<0.05),而与性别、年龄、病灶数、是否合并桥本甲状腺炎无关(P>0.05)。Spearman秩相关性分析显示,甲状腺乳头状癌中STAT3与NCK1的表达呈正相关(r=0.483,P<0.05);STAT3与VEGF的表达呈正相关(r=0.584,P<0.05);NCK1与VEGF的表达呈正相关(r=0.477,P<0.05)。结论STAT3、NCK1、VEGF存在协同作用,共同促进了甲状腺乳头状癌的血管生成和颈部淋巴结转移过程。
Objective To study the expression of STAT3,NCK1 and VEGF in papillary thyroid carcinoma and its clinical significance.Methods Sixty-three cases of papillary thyroid carcinoma,32 cases of thyroid adenoma,and 20 cases of normal thyroid tissue were collected from September 2019 to December 2020 in Tangshan Workers'Hospital.The expression levels of STAT3,NCK1 and VEGF were detected by immunohistochemistry.The expression of STAT3,NCK1 and VEGF in different thyroid tissues was analyzed,and the relationship between STAT3,NCK1 and VEGF in papillary thyroid carcinoma and clinical pathology was analyzed.Results The positive expression rates of STAT3,NCK1 and VEGF in papillary thyroid carcinoma were higher than those in thyroid adenoma and normal thyroid tissue,and the difference was statistically significant(P<0.05);while there was no significant difference in the positive expression rates of STAT3,NCK1 and VEGF between thyroid adenoma and normal thyroid tissues(P>0.05).STAT3 positive expression was correlated with tumor diameter and cervical lymph node metastasis(P<0.05),while was not related to gender,age,number of lesions,invasion of thyroid envelope,and hashimoto thyroiditis(P>0.05).The positive expression of NCK1 was correlated with cervical lymph node metastasis(P<0.05),while was not related to gender,age,tumor diameter,number of lesions,invasion of thyroid envelope,and hashimoto thyroiditis(P>0.05).The positive expression of VEGF was correlated with tumor diameter,invasion of thyroid envelope and cervical lymph node metastasis(P<0.05),was no correlation with gender,age,number of lesions and hashimoto thyroiditis(P>0.05).Spearman rank correlation analysis showed that STAT3 was positively correlated with NCK1 expression in papillary thyroid carcinoma(r=0.483,P<0.05);STAT3 was positively correlated with VEGF expression(r=0.584,P<0.05);NCK1 was positively correlated with VEGF expression(r=0.477,P<0.05).Conclusion STAT3,NCK1 and VEGF have a synergistic effect,which together promote the angiogenesis and cervical lymph node metastasis process of thyroid papillary carcinoma.
作者
李佳楠
陈春悠
LI Jia-nan;CHEN Chun-you(School of Stomatology,North China University of Science and Technology,Tangshan 063000,Hebei,China;Head and Neck Surgery,Tangshan Workers'Hospital,Tangshan 063000,Hebei,China)
出处
《医学信息》
2022年第5期94-97,共4页
Journal of Medical Information
作者简介
李佳楠(1996.4-),男,河北唐山人,硕士研究生,主要从事口腔颌面外科学研究;通讯作者:陈春悠(1962.1-),男,河北唐山人,硕士,教授,硕士生导师,主要从事头颈外科学的研究。
