摘要
目的探讨维生素A(VitA)、维生素D(VitD)、维生素K_(1)(VitK_(1))水平与儿童上呼吸道感染及免疫功能的相关性.方法选取2018年7月至2020年7月沈阳医学院附属中心医院收治的115例上呼吸道感染患儿(6个月~12岁)为研究组,选取同期在医院接受体检的95例健康儿童(5个月~12岁)为对照组,采用高效液相色谱法检测两组的血清VitA水平,采用液相串联质谱法检测血清VitD和VitK_(1)水平,利用细胞免疫芯片检测法分析CD4^(+)、CD8^(+)的T淋巴细胞亚群百分比及CD4^(+)/CD8^(+),并分析血清VitA、VitD和VitK_(1)水平与免疫功能的相关性.结果研究组的血清VitA、VitD水平均显著低于对照组(t值分别为8.149、10.069,P<0.05),研究组的血清VitA、VitD缺乏率均显著高于对照组(χ^(2)值分别为118.349、4.360,P<0.05),研究组与对照组血清VitK_(1)水平差异无统计学意义(P>0.05).研究组的CD4^(+)T淋巴细胞亚群百分比和CD4^(+)/CD8^(+)均显著低于对照组(t值分别为20.680、3.276,P<0.05);研究组VitA正常者CD4^(+)T淋巴细胞亚群百分比和CD4^(+)/CD8^(+)均显著高于VitA缺乏者(t值分别为5.728、5.554,P<0.05).经Pearson相关性分析显示,血清VitA与CD4^(+)T淋巴细胞亚群百分比和CD4^(+)/CD8^(+)均呈正相关(r分别为0.328、0.336,P<0.05).结论儿童上呼吸道感染的发生与VitA、VitD缺乏密切相关,与VitK_(1)关系不显著,其中VitA缺乏在上呼吸道感染患儿中还与免疫功能降低密切相关;在临床上应注意增加儿童必要的VitA和VitD摄入,避免因VitA或VitD水平不足造成的儿童机体免疫力下降,进一步增加上呼吸道感染的风险.
Objective To investigate the correlation of vitamin A(VitA),vitamin D(VitD)and vitamin K_(1)(VitK_(1))levels with upper respiratory tract infection and immune function in children.Methods 115 children(6 months to 12 years old)with upper respiratory tract infection admitted to the hospital from July 2018 to July 2020 were selected as the study group.Meanwhile,95 healthy children(5 months to 12 years old)who received physical examination in the hospital during the same period were selected as the control group.Serum VitA levels in the two groups were detected by high performance liquid chromatography,serumVitD and VitK_(1) levels were detected by liquid phase tandem mass spectrometry,Detection method of cellular immunity chip was used for quantitative analysis of the percentages of CD4^(+)T lymphocyte subsets,CD8^(+)T lymphocyte subsets and CD4^(+)/CD8^(+).The correlation between serum VitA,VitD and VitK_(1) levels and immune function-related indicators was analyzed.Results Serum VitA and VitD levels in the study group were significantly lower than those in the control group(t=8.149 and 10.069,respectively,P<0.05),and cases with serum VitA and VitD deficiency were significantly more in the study group than in the control group(χ^(2)=118.349 and 4.360,respectively,P<0.05).There was no statistically significant difference in VitK_(1) levels between the study group and the control group(P>0.05).The percentage of CD4^(+)T lymphocyte subsets and CD4^(+)/CD8^(+)in the study group were significantly lower than those in the control group(t=20.680 and 3.276,respectively,P<0.05).The percentage of CD4^(+)T lymphocyte subsets and CD4^(+)/CD8^(+)in children with normal VitA were significantly higher than those in children with VitA deficiency(t=5.728 and 5.554,respectively,P<0.05).Pearson correlation analysis showed that serum VitA was positively correlated with the percentage of CD4^(+)T lymphocyte subsets and CD4^(+)/CD8^(+)(r=0.328 and 0.336,respectively,P<0.05).Conclusion The occurrence of upper respiratory tract infection in children is closely related to VitA and VitD deficiency,but there is no significant correlation with VitK1.Besides,VitA deficiency is closely related to the decrease in immune function in children with upper respiratory tract infection.In clinical practice,attention should be paid to increasing children′s necessary intake of VitA and VitD,so as to prevent the decrease in immune function caused by VitA or VitD deficiency,which will increase the risk of upper respiratory tract infection.
作者
程双楠
徐浩
隋登宇
CHENG Shuangnan;XU Hao;SUI Dengyu(Department of Pediatrics,Central Hospital Affiliated to Shenyang Medical College,Liaoning Shenyang110024,China)
出处
《中国妇幼健康研究》
2022年第1期130-134,共5页
Chinese Journal of Woman and Child Health Research
关键词
维生素A
维生素D
维生素K
儿童上呼吸道感染
免疫功能
vitamin A
vitamin D
vitamin K1
upper respiratory tract infection in children
immune function
作者简介
程双楠(1987-),女,主治医师,硕士,主要从事儿科呼吸系统疾病的研究。;通讯作者:徐浩,主任医师。
