摘要
硫解酶(thiolase)是脂肪酸代谢的关键酶,在植物次生代谢合成、生长发育以及抵抗非生物胁迫中均发挥重要作用。为探究谷子硫解酶基因家族的基本特征,本研究利用生物信息学方法,对谷子硫解酶基因家族成员进行了鉴定,对蛋白理化性质及系统进化、基因结构、染色体位置、启动子、基因进化进行了分析;采用转录组测序进行了根、茎、叶、穗组织表达谱分析,运用实时荧光定量PCR(qRT-PCR)测定干旱胁迫下谷子硫解酶基因家族成员的表达水平。结果表明,谷子硫解酶基因家族共有5个成员,位于4条染色体上,编码的蛋白长度为401~461 aa,分子量为41~49 kDa,均含有硫解酶特有的2个保守基序。系统进化分析显示,谷子硫解酶基因家族分为2个亚家族(KAT和ACAT),KAT型硫解酶编码基因有14个外显子,ACAT型硫解酶有13个外显子。基因进化分析显示,谷子硫解酶基因既存在功能冗余也存在功能分化。谷子硫解酶编码基因启动子序列中含有光响应、激素类响应、胁迫诱导等多种顺式作用元件。谷子不同组织中硫解酶基因表达谱显示,KAT型硫解酶基因在根、茎、叶、穗部位均高表达;而ACAT型硫解酶基因仅在根和穗部位有较高的表达。干旱胁迫下,谷子硫解酶基因家族成员表达量均明显上调。本研究为揭示谷子硫解酶的功能及其在谷子抗旱育种中的应用提供了理论基础。
Thiolase,a key enzyme in fatty acid metabolism,was found to play an important role in plant secondary metabolic synthesis,growth and development,and abiotic stresses resistance.To investigate the basic characteristics of thiolase gene family in foxtail millet,the bioinformatic analysis were conducted to analyze the family members,the physicochemical properties and phylogenetic tree,gene structure,chromosome location,promoter,gene evolution.Expression profiling in root,stem,leaves and spike tissues was performed using transcriptome sequencing.The expressions of thiolase gene family under drought in foxtail millet were detected by quantitative real-time PCR.The results showed that thiolase gene family had 5 members in foxtail millet,which encoded proteins with 401~461 aa in length and 41~48 kDa in weight,contained two conserved motifs specific to thiolase,and were located on four chromosomes respectively.The result of phylogenetic tree showed that thiolase protein could be divided into two subfamilies(KAT and ACAT).The gene encoding the KAT-type thiolase had 14 exons and the ACAT-type thiolase had 13 exons.Gene evolutionary analysis revealed both functional redundancy and functional divergence.Promoter regions contained light-related elements and other cis-acting elements responding to hormones,abiotic stress.The expression of foxtail millet thiolase genes in different tissues showed KAT-type thiolase genes were highly expressed in roots,stems,leaves and spikes,while ACAT-type thiolase genes were specifically expressed at high level in roots and spikes.The relative expression of genes encoding thiolase were significantly up-regulated under drought stress.This study laid a foundation for further understanding the functions of thiolase,as well as breeding new foxtil millet varieties resistant to drought.
作者
姚锐
马鑫磊
顾鹏鹏
林小虎
高慧
YAO Rui;MA Xinlei;GU Pengpeng;LIN Xiaohu;GAO Hui(College of Agronomy and Biotechnologyu/Hebei Key Laboratory of Crop Stress Biology,Hebei Normal University of Science&Technology,Qinhuangdao,Hebei 066004)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第2期259-269,共11页
Journal of Nuclear Agricultural Sciences
基金
国家“十三五”重点研发计划(2019YFD1001701-2)
河北省现代农业产业技术体系创新团队(杂粮杂豆)项目(HBCT2018070404)
河北省自然基金资助项目(C2019407001)
河北省高等学校科学技术研究项目(QN2020154)。
关键词
谷子
硫解酶
基因家族
生物信息学
表达分析
foxtail millet
thiolase
gene family
bioinformatics
expression analysis
作者简介
姚锐,男,主要从事作物逆境分子生物学研究。E-mail:yaoruiha@163.com;通讯作者:林小虎,男,教授,主要从事作物种质资源研究。E-mail:xiaohulin2008@163.com;同为通讯作者:高慧,女,讲师,主要从事作物逆境胁迫研究。E-mail:gaohui1028@126.com。
