摘要
DW871是一个具有特异株型的甘蓝型油菜矮化品系。为了研究DW871的相关基因及其表达模式,我们以甘蓝型高秆油菜HW871为对照,选取抽薹期茎秆组织进行转录组基因表达分析。结果显示:DW871与HW871之间存在8665个显著差异基因,其中上调基因2582个,下调基因6083个。通过GO富集分析可注释到3个大类共22个亚类中。通过KEGG富集分析注释到5个大类31个亚类共295个通路中。我们从显著差异基因中筛选了与矮化性状密切相关的基因,在果胶降解代谢途径中注释到了43个差异基因;在生长素信号转导途径中注释到了69个差异基因;在木质素单体合成途径中注释到22个差异基因。发现DW871的矮化性状与细胞伸长、细胞壁形成和细胞分裂的异常以及生长素信号传导的受阻有关。尤其在木质素单体合成途径中的,可能通过调控PAL和CCR等酶的表达,改变木质素的合成速率和组成,进而对一系列与木质素相关的生理生化过程进行调控。因此提出DW871的矮化机制,即生长素应答和木质素合成的异常,造成了细胞伸长、生长和细胞壁变化的异常,并最终导致了DW871的矮化。
DW871 is a Brassica napus dwarf line with unusual and excellent plant type. To study the dwarf-related genes and their expression patterns of DW871, we took B. napus HW871 as control and selected stem tissues at the bolting stage for transcriptome gene expression analysis. Results showed that there were 8665 significant differentially expressed genes between DW871 and HW881, of which 2582 were up-regulated and 6083 down-regulated. The enrichment analysis of GO could be annotated to 22 subcategories in 3 categories, the enrichment analysis of KEGG was annotated to 295 pathways in 31 subcategories in 5 categories. We screened genes closely related to dwarfing traits from significantly different genes, 43 in pectin degradation, 69 in auxin signal transduction, and annotated 22 in the monolignol biosynthesis. It was found that the dwarfing character of DW871 was related to cell elongation, abnormal cell wall formation and cell division, and obstruction of auxin signal transduction. In particular, in monolignol biosynthesis, it is possible to regulate a series of physiological and biochemical processes related to lignin by regulating the expression of enzymes such as PAL and CCR. The differential genes related to dwarfing traits were screened in this research. It could be hypothesized that the dwarfing mechanism of DW871 involving abnormal auxin response and lignin synthesis,which lead to abnormal cell elongation,growth and cell wall changes,and ultimately lead to the dwarfing of DW871.
作者
罗京
李超
张瑞茂
赵德刚
高志宏
王芳
杨元雨
王转转
王敏
LUO Jing;LI Chao;ZHANG Rui-mao;ZHAO De-gang;GAO Zhi-hong;WANG Fang;YANG Yuan-yu;WANG Zhuan-zhuan;WANG Min(School of Life Sciences,Guizhou Normal University,Guiyang 550025,China;Guizhou Oil Research Institute,Guizhou Academy of Agriculture Sciences,Guiyang 550006,China;Guizhou Rapeseed Institute,Guizhou Academy of Agriculture Sciences,Guiyang 550006,China;Key Laboratory of Plant Resources Conservation and Germplasm Innovation Center for Mountainous Region(Ministry of Education),Collaborative Innovation Center for Mountain Ecology&Agro-Bioengineering,Institute of Agro-Bioengineering/College of Life Sciences,Guizhou University,Guiyang 550025,China)
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2022年第1期14-24,共11页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(32060464)
贵州省科学技术基金重点项目(黔科合基础[2017]1415)
贵州省人才基地(RCJD2018-14)
贵州省植物保育技术应用工程研究中心
中央引导地方科技发展专项资金(黔科中引[2018]4001)
贵州省农业科学院项目(黔农科院种质资源[2021]05号)。
作者简介
罗京(1996-),男,湖南岳阳人,硕士研究生,研究方向为植物生化化学与分子生物学,E-mail:anrymeng0@hotmail.com;通讯作者:李超(1977-),男,苗族,贵州道真人,研究员,博士,研究方向为油菜遗传育种,E-mail:gzlichao@126.com。
