摘要
为制备酒糟腐熟复合微生物菌剂用于生产生物有机肥,对筛选得到的贝莱斯芽孢杆菌诱变育种菌株UN-5和里氏木霉的复合效果进行了考察。针对菌种耐酸性、复合时二者拮抗性以及培养时间、菌种配比和培养pH这3个影响复合菌剂纤维素酶活的主要因素设计单因素及正交实验。结果表明,复合菌剂的菌种配比、培养时间和pH对复合菌剂的纤维素酶活有显著影响。正交实验结果显示UN-5和里氏木霉制备复合菌剂的最佳条件为:培养时间28 h,菌种配比(UN-5∶里氏木霉)为2.5∶1,培养pH为5.0,此条件下制备的复合菌剂纤维素酶活达125.24 U,有效活菌数为10.3亿/g。
In order to prepare the compound microbial agent for distiller’s grains to produce bio-organic fertilizer,the compound effect of mutation breeding strain UN-5 and Trichoderma reesei was investigated. Single factor and orthogonal experiments were designed according to the main factors such as acid resistance,antagonistic resistance,culture time,strain ratio and pH. The results showed that the ratio of bacteria,culture time and pH had significant influence on the cellulase activity. The results of orthogonal experiment showed that the optimal conditions for the preparation of the composite microbial agent were as follows:the culture time was 28 h,the strain ratio(UN-5∶Trichoderma reesei)was 2.5∶1,and the culture pH was 5.0. Under these conditions,the cellulase activity was 125.24 U and the effective viable number was 10.3 billion per gram.
作者
马倩
何颂捷
陈静
左勇
秦世蓉
宋华
胡琨
MA Qian;HE Song-jie;CHEN Jing;ZUO Yong;QIN Shi-rong;SONG Hua;HU Kun(College of Life Science,Sichuan Normal University,Chengdu Sichuan 610101;Zigong Inspection Testing Institute,Zigong Sichuan 643200;Soild-state Fermentation Resource Utilization Key Laboratory of Sichuan,Yibin Sichuan 644000;Sichuan Shiliwang Agriculturel Technology Development Co.,Ltd.,Yibin Sichian 644000)
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2022年第1期213-219,共7页
Soil and Fertilizer Sciences in China
基金
四川省科技厅重点研发项目(2018NZ0050)
固态发酵资源利用四川省重点实验室应用研究项目(2018GTY004)
宜宾市科技计划项目(2018NY009)
四川轻化工大学研究生创新基金(Y2018061)。
关键词
酒糟
生物有机肥
微生物菌剂
正交实验
distiller’s grains
biological organic fertilizer
microbial inoculum
orthogonal test
作者简介
马倩(1997-),硕士研究生,研究方向为微生物。E-mail:maqianle12@163.com;通讯作者:左勇,E-mail:sgzuoyong@tom.com。
