摘要
采用形态学与分子生物学技术对草莓根腐病病原菌进行鉴定。对草莓育苗圃内发病草莓根部进行组织培养、病原菌分离纯化,通过传统的形态学观察初步判断该病原菌菌株为土赤壳属病原菌。利用通用引物ITS1/ITS4初步鉴定该病原菌菌株ZJ16属于土赤壳属,但不能鉴定到种,又选择土赤壳属特异基因EF-1α和Histone H3基因引物序列进行PCR扩增分析,并将待测菌株测序结果与GenBank中相关菌株EF-1α和Histone H3序列进行同源性比较,待测菌株与Ilyonectria novozelandica(GenBank收录号:CBS 112593)的同源性均达到了100%。确定新疆地区草莓根腐病病原菌为Ilyonectria novozelandica,该菌种是1个中国新记录种。
Identification on strawberry root rot pathogen was conducted by morphology and molecular biology technique.Tissue culture,pathogen isolation and purification of sicken strawberry roots in strawberry breeding nursery was carried out.The pathogen strain was initially diagnosed as Ilyonectria pathogen by traditional morphological observation.The ZJ16 strain was initially identified by universal primers ITS1/ITS4.It was belonging to the genus Ilyonectria,which could not be identified as species.The specific gene EF-1αand Histone H3 primer sequence were selected and analyzed by PCR amplification.Homology comparison between sequencing result of waiting tested strain and related strain EF-1αand Histone H3 sequence in GenBank were conducted.The homology of waiting tested strain and Ilyonectria novozelandica(CBS 112593)were all 100%.It was confirmed that strawberry root rot pathogen in Xinjiang region was identified as Ilyonectria novozelandica,which was a new recorded species in China.
作者
史芳芳
褚继萍
SHI Fangfang;CHU Jiping(Agricultural Scientific Institute of the 12th Division of Xinjiang Production and Construction Corps,Urumchi 830088,Xinjiang,China)
出处
《中国蔬菜》
北大核心
2022年第2期95-100,共6页
China Vegetables
基金
兵团中青年科技创新领军人才计划项目(2018CB030)。
关键词
草莓根腐病
土赤壳属
多基因系统进化分析
病原鉴定
strawberry root rot
Ilyonectria
multigene phylogenetic analysis
pathogen identification
作者简介
史芳芳,女,高级农艺师,主要从事草莓育种和病害鉴定工作,E-mail:451784039@qq.com;通信作者:褚继萍,女,高级农艺师,主要从事果树栽培研究,E-mail:gzx120731@sina.com。
