摘要
目的建立焦磷酸测序对药品中5种致病菌的快速鉴定方法。方法通过信息网站及专业设计软件设计引物,扩增后进行电泳与焦磷酸测序,测序结果与Genebank库相应基因序列进行比对。结果5种致病菌测序结果与Genebank库相应基因序列匹配率均在93%以上,均能得到良好的鉴定结果。结论焦磷酸测序方法可以实现对5种致病菌的快速鉴定,大大提高鉴定效率。
Objective To establish a rapid identification method for five pathogenic bacteria in drugs by pyrosequencing.Methods Primers were designed through information website and professional design software,and then electrophoresis and pyrosequencing were conducted after the amplification of primers.The sequencing results were compared with the corresponding gene sequences in Genebank library.Results The sequencing results of the five pathogenic bacteria matched the corresponding gene sequences of the Genebank library by more than 93%,and all of them could be identified well.Conclusion The method of pyrosequencing can realize the rapid identification of five pathogenic bacteria and greatly improve the identification efficiency.
作者
李秀珍
刘风琴
王芳
郭树静
LI Xiuzhen;LIU Fengqin;WANG Fang;GUO Shujing(Department of Biological Testing,Dezhou Food and Drug Inspection and Testing Center,Shandong,Dezhou 253000,China)
出处
《中国医药科学》
2022年第1期55-58,共4页
China Medicine And Pharmacy
关键词
焦磷酸测序
5种致病菌
通用引物
快速鉴定
Pyrosequencing
Five pathogenic bacteria
Universal primer
Rapid identification
