摘要
目的:探讨葛根素调节钙调素依赖性蛋白激酶Ⅱ(CaMKⅡ)对布比卡因中毒大鼠心肌损伤的影响。方法:将雄性Wistar大鼠40只采用随机数字表法分为空白对照组(C组)、布比卡因中毒模型组(B组)、葛根素预防组(P组)、葛根素治疗组(T组),每组10只。C组经股静脉输注0.9%生理盐水3 mL/kg;B组经股静脉输注0.5%布比卡因10 mg/kg,出现心律失常后输注0.9%生理盐水3 mL/kg;P组经股静脉输注葛根素3 mL/kg 5 min后输注0.5%布比卡因10 mg/kg;T组经股静脉输注0.5%布比卡因10 mg/kg,出现心律失常后立即输注葛根素3 mL/kg。C组在开始输注生理盐水的第8 min及B、T、P组在开始输注利多卡因的第8 min,处死大鼠。取左室心肌组织,采用流式细胞仪测定心肌细胞Ca^(2+)浓度,Western blot法测定心肌细胞CaMKⅡ磷酸化水平,TUNEL染色观察细胞凋亡情况。结果:与C组比较,B组心肌细胞CaMKII磷酸化水平、Ca^(2+)浓度、细胞凋亡率均升高(P<0.05)。与B组比较,P组心肌细胞CaMKII磷酸化水平、Ca^(2+)浓度、细胞凋亡率均降低(P<0.05),T组心肌细胞CaMKⅡ磷酸化水平、Ca^(2+)浓度、细胞凋亡率均降低,但差异无统计学意义(P>0.05)。结论:葛根素可能通过调节CaMKⅡ来减轻布比卡因中毒大鼠的心肌损伤。
Objective:To investigate the effect of puerarin on myocardial damage in bupivacaine intoxicated rats via regulating CaMKⅡ.Methods:Forty male Wistar rats were allocated to blank control group(C group),bupivacaine intoxicated model group(B group),puerarin prevention group(P group)and puerarin treatment group(T group)using random number table method,with ten rats in each group.C group was infused with 0.9%physiological saline,3 mL/kg,through femoral vein;B group was infused with 0.5%bupivacaine,10 mg/kg,through femoral vein,and 0.9%physiological saline,3 mL/kg,when cardiac arrhythmia occurred;P group with puerarin,3 mL/kg,through femoral vein,and 0.5%bupivacaine,10 mg/kg,in five minutes;T group with 0.5%bupivacaine,10mg/kg,through femoral vein,and puerarin,3 mL/kg,instantly when cardiac arrhythmia occurred.The rats were sacrificed at the eighth minute when C group began to infuse physiological saline,B group,T group and P group began to infuse lidocaine.To detect the concentrations of myocardial Ca^(2+)using flow cytometry after taking left ventricular myocardial tissue,to detect phosphorylation level of myocardial CaMKⅡusing Western blot method,to observe cellular apoptosis using TUNEL staining.Results:Compared with C group,phosphorylation level of myocardial CaMKⅡ,the concentrations of Ca^(2+)and cellular apoptosis rate of B group rose(P<0.05).Compared with B group,phosphorylation level of myocardial CaMKⅡ,the concentrations of Ca^(2+)and cellular apoptosis rate of P group decreased(P<0.05),phosphorylation level of myocardial CaMKⅡ,the concentrations of Ca^(2+)and cellular apoptosis rate of T group lowered,while the difference had no statistical meaning(P>0.05).Conclusion:Puerarin could relieve myocardial damage in bupivacaine intoxicated rats possibly through regulating the levels of CaMKⅡ.
作者
于妍
王春爱
李玉兰
梁曦
温晓辉
YU Yan;WANG Chun'ai;LI Yulan;LIANG Xi;WEN Xiaohui(Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;The First Hospital of Lanzhou University;Gansu University of Chinese Medicine)
出处
《西部中医药》
2022年第1期29-31,共3页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81760892)。
作者简介
于妍(1983-),女,博士学位,主治医师。研究方向:心血管疾病及心肌病的中西医结合诊疗;通讯作者:王春爱(1970-),女,硕士学位,主任医师。研究方向:临床麻醉与镇痛。
