摘要
研究防褐化处理对蒙古栎嫩枝扦插生根的影响。以蒙古栎3年实生苗的嫩枝为材料,分别采用乙醇、硝酸银、抗坏血酸对插穗进行防褐化处理,采用500 mg·L^(-1) IBA-K浸蘸处理后扦插,并分别在扦插后0、10、20、30、40、50 d取样测定不同生根时期氧化蛋白酶活性和内源激素含量。结果表明,防褐化处理可有效提高扦插生根率,以1%乙醇1 h+500 mg·L^(-1) IBA-K处理2 h最佳,生根率、根系效果指数分别为21.1%和5.713。在生根过程中,外施500 mg·L^(-1) IBA-K能够提升插穗内POD、PPO的活性,降低IAAO的活性,且IAA/ABA、IAA/ZR的比值升高,有利于蒙古栎嫩枝扦插生根。由此说明1%乙醇1 h浸泡结合500 mg·L^(-1) IBA-K激素处理,通过提高POD与PPO的活性,可降低IAAO的活性,增加IAA/ZR和IAA/ABA的比值,提升蒙古栎嫩枝扦插成活率。
The effects of the anti-browning treatment on the cutting rooting of Quercus mongolica were studied.The twigs of the 3-year-old seedlings of Q.mongolica were used as materials.Ethanol,silver nitrate,and ascorbic acid were used to conduct the anti-browning treatment,and the cutting were carried out after the dip treatment with 500 mg/L indole-3-butyric acid potassium salt(IBA-K).Meanwhile,samples were taken at 0,10,20,30,40,and 50 days after cutting to determine the oxidative protease activity and endogenous hormone content at different rooting stages.The anti-browning treatment could effectively increase the rooting rate of the cuttings.Among them,the treatment with 1%ethanol for 1h and 500 mg/L IBA-K for 2 h was the best.The rooting rate and the root effect index were 21.1%and 5.713,respectively.During the rooting process,the external application of 500 mg/L IBA-K could increase the activity of POD and PPO in the cuttings,reduce the activity of IAAO,and increase the ratio of IAA/ABA and IAA/ZR,which were beneficial to cuttings.By means of increasing the activity of POD and PPO,dipping with 1%ethanol for 1 h in combination with 500 mg/L IBA-K hormone treatment can reduce the activity of IAAO,increase the ratio of IAA/ZR and IAA/ABA,and improve the cutting survival rate.
作者
虞家昕
战昊
王思进
王天宠
陆秀君
YU Jia-xin;ZHAN Hao;WANG Si-jin;WANG Tian-chong;LU Xiu-jun(College of Forestry,Shenyang Agricultural University,Shenyang 110866,Liaoning,China)
出处
《西北林学院学报》
CSCD
北大核心
2022年第1期119-124,229,共7页
Journal of Northwest Forestry University
基金
国家重点研发计划课题(2017YFD0600602)。
关键词
蒙古栎
嫩枝扦插
防褐化
氧化蛋白酶活性
内源激素
Quercus mongolica
twig cutting
anti-browning
oxidative protease activity
endogenous hormone
作者简介
第一作者:虞家昕,研究方向:森林培育,E-mail:yujiaxinshenyang@foxmail.com;通信作者:陆秀君,教授,博士生导师,研究方向:森林培育,E-mail:lxjsyau@syau.edu.cn。
