摘要
【目的】研究瘦素(LEP)对猪原代脂肪细胞脂滴相关基因FITM2、PLIN1和PLIN2 mRNA表达的影响。【方法】采用不同质量浓度的LEP(0、50、100和150 ng/mL)处理15日龄三元杂交猪(DLY)皮下原代脂肪细胞48 h,试剂盒测定细胞中甘油三酯(TAG)含量,qPCR检测脂滴相关基因mRNA表达水平。【结果】与0 ng/mL LEP相比,100 ng/mL LEP可显著增加猪原代脂肪细胞TAG含量以及FITM2和PLIN2 mRNA表达水平(P<0.05),显著下调PLIN1 mRNA表达水平(P<0.05);与50和100 ng/mL LEP相比,150 ng/mL LEP可显著降低脂肪细胞TAG含量(P<0.05),显著上调PLIN1和PLIN2 mRNA表达水平(P<0.05)。【结论】LEP可促进猪原代脂肪细胞FITM2和PLIN2 mRNA表达,可促进或抑制PLIN1 mRNA表达,但LEP对脂滴相关基因表达的促进或抑制存在质量浓度差异性。
[Purpose]To study the effect of leptin(LEP)on the mRNA expression of lipid droplet related genes FITM2,PLIN1 and PLIN2 in porcine primary adipocytes.[Methods]Subcutaneous primary adipocytes from DLY pigs at 15-day was treated with different mass concentrations LEP including 0,50,100 and 150 ng/mL for 48 hours,and the content of triglyceride(TAG)in cells were measured,and the mRNA expression levels of lipid droplet related genes FITM2,PLIN1 and PLIN2 were detected using qPCR technology.[Results]Compared with 0 ng/mL LEP,the content of TAG and the mRNA expression levels of FITM2 and PLIN2 were significantly increased in 100 ng/mL LEP(P<0.05),the mRNA expression level of PLIN1 was significantly down-regulated(P<0.05).TAG content of 150 ng/mL LEP was significantly lower than 50 and 100 ng/mL LEP(P<0.05),and the mRNA expression levels of PLIN1 and PLIN2 were significantly higher than 50 and 100 ng/mL LEP(P<0.05).[Conclusion]LEP can promote the mRNA expression of FITM2 and PLIN2,it can promote or inhibit the mRNA expression of PLIN1,however,there are differences in the mass con-centration of LEP in promoting or inhibiting the expression of lipid droplet related genes.
作者
何永江
肖慧
黄英
杨明华
赵素梅
潘洪彬
刘琛
彭泽琴
HE Yongjiang;XIAO Hui;HUANG Ying;YANG Minghua;ZHAO Sumei;PAN Hongbin;LIU Chen;PENG Zeqin(Faculty of Animal Science and Technology,Yunnan Agricultural University,Yunnan Key Laboratory of Animal Nutrition and Feed,Kunming 650201,China;Diqing Tibetan Autonomous Prefecture’s Secondary Vocational School for Nationalities,Diqing 674499,China;College of Biotechnology and Engineering,West Yunnan University,Lincang 677000,China)
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第1期91-96,共6页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省应用基础研究面上项目(2017FB068)。
作者简介
何永江(1997-),男,四川泸州人,在读硕士研究生,主要从事动物营养与饲料科学研究。E-mail:1334360977@qq.com;通信作者:彭泽琴(1979-),女,云南临沧人,硕士,讲师,主要从事动物营养及饲养研究。E-mail:53748551@qq.com。
