摘要
[目的]确定内蒙古锡林郭勒盟多伦县某养殖场犊牛腹泻的致病菌,为选择敏感药物治疗该病提供依据。[方法]无菌采集腹泻犊牛肛拭子进行细菌培养鉴定、致病性试验及体外药物敏感试验。[结果]肛拭子经培养后的菌落形态一致,经表型鉴定、生理生化鉴定及16S rDNA鉴定确定分离菌为大肠杆菌;大肠杆菌分型引物鉴定分离菌株为ETEC型;且分离菌株可致小鼠死亡;分离菌株对氨苄西林、氨曲南、头孢唑啉、四环素、氧氟沙星、左氧氟沙星、复方新诺明等21种药物表现为耐药,对头孢哌酮、头孢曲松、克林霉素、多黏菌素B、米诺环素等10种药物表现为中度敏感,对头孢西丁、万古霉素、环丙沙星及呋喃妥因4种药物表现为敏感。[结论]该养殖场的犊牛腹泻是由大肠杆菌ETEC型所致,临床治疗过程中可使用环丙沙星。
[Objective]To determine the pathogenic bacteria causing calf diarrhea in a cattle farm in Duolun County,Xilin Gol League,Inner Mongolia,and to provide a basis for selecting sensitive drugs to treat this disease.[Method]Anal swabs from calves with diarrhea were collected aseptically for bacterial isolation,identification,pathogenicity test and in vitro antimicrobial sensitivity test.[Result]The bacterial strains isolated from the anal swab samples had the consistent colony morphology.The isolates were confirmed as Escherichia coli(E.coli)by phenotypic identification,physiological and biochemical identification and 16S rDNA gene sequencing identification.The isolates were molecularly identified as ETEC type by using the E.coli typing primers.In addition,the E.coli isolates could cause mice death.The E.coli isolats were resistant to 21 antimicrobial agents such as ampicillin,aztreonam,cefazolin,tetracycline,ofloxacin,levofloxacin and compound trimethoprim,were moderately sensitive to 10 antimicrobial agents such as cefoperazone,ceftriaxone,clindamycin,polymyxin B and minocycline,and were sensitive to 4 antimicrobial agents such as cefoxitin,vancomycin,ciprofloxacin and nitrofurantoin.[Conclusion]The calf diarrhea in this cattle farm was caused by enterotoxigenic E.coli.Ciprofloxacin was recommended to be used in treatment of this disease.
作者
王娜
戴伶俐
乌云塔娜
钢托亚
赵世华
达来宝力格
WANG Na;DAI Ling-li;Wuyuntana;Gangtuoya;ZHAO Shi-hua;Dalaibaolige(Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot010031,China;Animal Disease Prevention and Control Center of New Barag Right Banner of Hulun Buir City,New Barag Right Banner021300,China)
出处
《畜牧与饲料科学》
2021年第6期106-111,共6页
Animal Husbandry and Feed Science
基金
内蒙古农牧业创新基金项目(2021CXJJM04)
内蒙古自治区科技重大专项(2020ZD0006)。
关键词
大肠杆菌
犊牛
腹泻
分离鉴定
Escherichia coli
calf
diarrhea
isolation and identification
作者简介
王娜(1989-),女,助理研究员,硕士,主要研究方向为动物传染病防控;通信作者:达来宝力格(1979-),男,助理研究员,博士,主要研究方向为动物传染病防控。
