摘要
目的对HIV感染待确定样本进行复检、确证,为艾滋病防治工作提供依据。方法对2016—2020年泰州市各艾滋病实验室上送的HIV感染待确定样本,采用胶体硒法和酶联免疫吸附法进行复检,复检阳性的样本再用免疫印迹法确证。结果1460份HIV感染待确定样本经过复检,有1133份阳性;经免疫印迹法确证,有1018份为HIV-1抗体阳性,确证阳性率为69.73%,不确定55份,阴性60份。确证阳性率最高的送检单位是疾控机构,采供血机构最低;55份不确定样本中有36例成功随访,其中21例转为阳性。结论不同机构送检样本的确证阳性率有较大差异,对HIV-1抗体不确定病例应加强随访,必要时做核酸检测。
Objective To analyze the reexamination and confirmatory test results of HIV undetermined infection samples,so as to provide basis for AIDS prevention and control.Methods From 2016 to 2020,HIV undetermined infection samples collected from Taizhou AIDS laboratories were reexamined by colloidal selenium method and enzyme-linked immunosorbent assay,and the positive samples were confirmed by Western blot.Results A total of 1133 of 1460 HIV infected undetermined samples were positive after reexamination test.After Western blot test,1018 samples were HIV-1 antibody positive,the confirmatory positive rate was 69.73%,55 were uncertain,and 60 were negative.The highest confirmatory positive rate samples were from centers for disease prevention and control,and the lowest ones were from blood banks.Among 55 uncertain samples,36 cases were followed up successfully and 21 cases turned to positive.Conclusion There was significant difference in the positive rate of samples from different institutions.For cases with uncertain HIV-1 antibody detection,follow-up testing should be strengthened,and nucleic acid test should be conducted.
作者
魏爱旺
陆祥彬
马智龙
WEI Ai-wang;LU Xiang-bin;MA Zhi-long(Taizhou Center for Disease Control and Prevention,Taizhou,Jiangsu 225300,China)
出处
《中国国境卫生检疫杂志》
CAS
2021年第6期416-418,共3页
Chinese Journal of Frontier Health and Quarantine
关键词
HIV抗体
艾滋病
筛查
免疫印迹试验
HIV-antibody
Acquired immunodeficiency syndrome
Screen
Western blotting test
作者简介
通信作者:魏爱旺,E-mail:tzcdclab@163.com。
