摘要
【目的】分离鉴定赣南地区番茄青枯病菌,明确菌系分化,为当地番茄抗青枯病育种和病害防治奠定基础。【方法】从江西省赣南地区采集番茄青枯病病株,经选择性平板分离、纯化和分子鉴定,获得不同地理来源的青枯菌Ralstonia solanacearum菌株。通过生理生化测定和接种番茄试验,鉴定青枯菌的生化变种和致病类型。PCR扩增内切葡聚糖酶基因egl序列,明确青枯菌的演化型和序列变种。双层平板培养法测定其对8个不同噬菌体的敏感性。【结果】获得了来自赣南地区9个市(县)的番茄青枯菌菌株44个,其中,41个菌株为生化变种Ⅲ,3个菌株为生化变种Ⅳ;致病力测定结果聚为Ⅰ、Ⅱ和Ⅲ类,其致病力分别为强、中和弱,其中,强致病力菌株占65.9%。所有菌株属于亚洲分支演化型(Ⅰ),并进一步划分为Sequevar13、14、15、17、18、34、44和48等8个序列变种。大部分菌株对供试的8个噬菌体敏感。【结论】赣南地区番茄青枯菌以生化变种Ⅲ和强致病力菌株为主,对噬菌体较敏感,存在8个序列变种,具有明显的菌系分化现象和遗传多样性。
【Objective】Isolating and identifying Ralstonia solanacearum strains from tomato plants in the Southern of Jiangxi Province,and clarifying the bacterial differentiation can lay the foundation for local tomato bacterial wilt resistance breeding and disease control.【Method】The diseased tomato plants were collected from the south of Jiangxi Province,R.solanacearum strains with different geographical origins were isolated by selective plate,purificated and identificated by PCR.The test of physiology and biochemistry and inoculation on tomato plants were conducted for the determination of biovar and virulence difference.The endoglucanase gene(egl)fragments were amplified by PCR to determine the phylotype and sequevar of R.solanacearum.【Result】A total of 44 R.solanacearum strains were obtained from nine cities(counties)in the south of Jiangxi Province,among which 41 strains were identified as biovar Ⅲ and three strains were identified as biovar Ⅳ.According to the results of virulence difference,44 strains were clustered into three groups,namely group Ⅰ(high virulence),group Ⅱ(moderate virulence)and group Ⅲ(weak virulence),of which group Ⅰ(high virulence)strains accounted for 65.9%.All strains were belonged to the phylotype Ⅰ and further divided into eight sequevars,namely Sequevar 13,14,15,17,18,34,44 and 48 respeclively.Most R.solanacearum strains were sensitive to the eight tested bacteriophages.【Conclusion】The strains of R.solanacearum from tomato in the south of Jiangxi Province are mainly biovar Ⅲ and high virulence,sensitive to bacteriophages,have eight sequevars,and have obvious differentiation and genetic diversity.
作者
任敏华
张静燕
崔晓东
陈荣华
刘琼光
REN Minhua;ZHANG Jingyan;CUI Xiaodong;CHEN Ronghua;LIU Qiongguang(College of Plant Protection,South China Agricultural University,Guangzhou 510642,China;Institute of Agricultural Sciences in Ganzhou,Ganzhou 341000,China;Key Laboratory of Microbial Signals and Crop Disease Control of Guangdong Province,Guangzhou 510642,China)
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2022年第1期67-76,共10页
Journal of South China Agricultural University
基金
江西省赣州市科技项目(赣市财教字[2019]号)。
作者简介
任敏华,硕士研究生,主要从事植物细菌病害研究,E-mail:renmishka@163.com;通信作者:陈荣华,研究员,主要从事农作物病害研究,E-mail:Chenronghua009@163.com;刘琼光,副教授,博士,主要从事植物细菌病害研究,E-mail:qgliu@scau.edu.cn。
