摘要
目的探讨LncRNA CRNDE和甲状腺乳头状癌(PTC)之间的关系。方法采用T检验、Kruskal-Wallis检验、Wilcoxon秩和检验和卡方检验评估临床病理特征与CRNDE表达的关系。受试者工作特征(ROC)曲线下面积(AUC)描述CRNDE对PTC的敏感性及特异性。Kaplan-Meier法和Cox回归分析评估影响PTC预后的因素;免疫浸润分析描述CRNDE与免疫细胞的相关性。结果PTC中CRNDE表达增加,BRAF V600E突变与PTC中CRNDE高表达相关。ROC分析表明CRNDE具有显著诊断能力(AUC=0.8397)。CRNDE高表达的总生存(OS)较差(P=0.0126),且CRNDE表达与PTC患者的OS独立相关(HR=2.131;P=0.0016)。CRNDE的表达与B细胞、CD8;T细胞及巨噬细胞的浸润水平呈正相关(P<0.05),与CD4;T细胞的浸润水平呈负相关(P<0.0001)。结论CRNDE的表达与PTC的生存率和免疫浸润显著相关,其可能是PTC中一种有前景的预后生物标志物。
Objective To investigate the relationship between LncRNA CRNDE and thyroid papillary carcinoma(PTC). Methods T-test, Kruskal-Wallis test, Wilcoxon rank-sum test and Chi-square test were used to evaluate the relationship between clinicopathological characteristics and CRNDE expression. The sensitivity and specificity of CRNDE to PTC were described by area under receiver operating characteristic(ROC) curve(AUC). Kaplan-Meier method and Cox regression analysis were used to evaluate factors that affect prognosis. The correlation between CRNDE and immune cells was described by immunoinfiltration analysis. Results CRNDE expression was increased in PTC, and BRAF V600 E mutation was associated with high CRNDE expression in PTC. ROC analysis showed that CRNDE had significant diagnostic ability(AUC=0.839 7). The overall survival(OS) of high CRNDE expression was poor(P=0.012 6), and the expression of CRNDE was independently correlated with the overall survival(OS) of PTC patients(HR=2.131;P=0.001 6). The expression of CRNDE was positively correlated with the infiltration level of B cells, CD8;T cells and macrophages(P<0.05), and negatively correlated with the infiltration level of CD4;T cells(P<0.000 1). Conclusion The expression of CRNDE is significantly related to the survival rate and immune infiltration in PTC, and it may be a promising prognostic biomarker in PTC.
作者
居悦俊
沈婷
陈刚
孔颖宏
JU Yuejun;SHEN Ting;CHEN Gang;KONG Yinghong(Department of Endocrinology,the Second People′s Hospital of Changshu in Jiangsu Province,Changshu,Jiangsu,215500;Ultrasonic Department,the Second People′s Hospital of Changshu in Jiangsu Province,Changshu,Jiangsu,215500)
出处
《实用临床医药杂志》
CAS
2021年第24期20-24,共5页
Journal of Clinical Medicine in Practice
基金
江苏省苏州市科技发展计划(民生科技-医疗卫生应用基础研究)项目(SYSD2019015)
江苏省常熟市卫生健康委员会指导性项目(cswzd202019)
江苏省常熟市卫生健康委员会资助性重点项目(csws202009)。
作者简介
通信作者:孔颖宏,E-mail:kongyinghong@163.com。
