摘要
目的:探究丹参酮提取物对阿尔茨海默病模型大鼠的干预效果及作用机制。方法:选取40只SD健康雄性大鼠,随机选取10只作为正常组,其余30只建立阿尔兹海默病模型,并分为模型组、低剂量组、高剂量组。低剂量组、高剂量组大鼠分别使用10 g/L、30 g/L丹参酮提取物溶液进行灌胃,正常组、模型组大鼠使用0.9%氯化钠溶液进行灌胃。对各组大鼠学习记忆能力进行测试,酶联免疫吸附实验法检测白细胞介素(IL)-4、高敏C反应蛋白(hs-CRP)、肿瘤坏死因子-α(TNF-α)水平,流式细胞仪检测各组大鼠海马区细胞凋亡情况,Western blotting法检测Bcl-2、Bax、caspase-3表达。结果:模型组逃避潜伏期长于正常组(P<0.01),穿越平台次数低于正常组(P<0.01);高剂量组大鼠逃避潜伏期低于模型组和低剂量组(P<0.01),穿越平台次数多于模型组和低剂量组(P<0.01和P<0.05)。hs-CRP、TNF-α、IL-4水平由高到低的排序依次均为:模型组、高剂量组、低剂量组和正常组(P<0.01)。4组之间不同时间点海马区细胞凋亡率由高到低的排序依次为:模型组、低剂量组、高剂量组和正常组(P<0.01)。Bcl-2的表达量在4组之间由高到低的排序依次为:正常组、高剂量组、低剂量组和模型组(P<0.01);Bax与caspase-3的表达量在4组之间由高到低的排序依次均为:模型组、低剂量组、高剂量组和正常组(P<0.01)。结论:使用丹参酮提取物对阿尔兹海默病模型大鼠进行干预,能够提升阿尔兹海默病大鼠学习记忆能力,减轻脑组织炎症反应,调控细胞凋亡相关蛋白Bcl-2、Bax、caspase-3相对表达量,抑制阿尔兹海默病大鼠海马区细胞凋亡,具有一定神经元保护作用。
Objective:To explore the intervention effects of tanshinone extract on Alzheimer′s disease model rats and its mechanism.Methods:Forty healthy male SD rats were randomly divided into the normal group,model group,low-dose group and high-dose group(10 rats in each group).The low-dose group and high-dose group were given 10 g/L and 30 g/L tanshinone extract by intragastric administration,respectively.The normal group and model group were given 0.9%sodium chloride solution by intragastric administration.The learning and memory ability in each group were tested.The levels of interleukin4(IL-4),high-sensitivity C-reactive protein(hs-CRP)and tumor necrosis factor-α(TNF-α)were detected using enzyme-linked immunosorbent assay,the apoptosis of hippocampal cells in each group was detected using flow cytometry,and the protein expression levels of Bcl-2,Bax and caspase-3 were detected using Western blotting.Results:The latency of escape in model group was longer than that in normal group(P<0.01),and the number of crossing platform in model group was lower than that in normal group(P<0.01).The escape latency in high-dose group was lower than that in model group and low-dose group(P<0.01),and the number of crossing platform in high-dose group was more than that in model group and low-dose group(P<0.01 and P<0.05).The order of hs-CRP,TNF-αand IL-4 levels from high to low was as follows:model group,high-dose group,low-dose group and normal group(P<0.01).The order of apoptosis rate of hippocampal cells at different time points was as follows:model group,low-dose group,high-dose group and normal group(P<0.01).The order of Bcl-2 expression in the four groups from high to low was the normal group,high-dose group,low-dose group and model group(P<0.01).The expression levels of Bax and caspase-3 were in descending order among the four groups:model group,low-dose group,high-dose group and normal group(P<0.01).Conclusions:The intervention of tanshinone extract on Alzheimer′s disease model rats can improve the learning and memory ability,alleviate the inflammatory reaction of brain tissue,regulate the relative expression of apoptosis-related proteins,Bcl-2,Bax and caspase-3,and inhibit the apoptosis of hippocampal cells,which has certain neuronal protection effect in Alzheimer′s disease rats.
作者
李志海
杨勤珍
木崇仙
LI Zhi-hai;YANG Qin-zhen;MU Chong-xian(Department of Neurology,Yunnan Geriatrics Hospital,Kunming Yunnan 650011;Department of Psychiatry,Yunnan Provincial Hospital for Infectious Diseases,Kunming Yunnan 650301;Department of Neurology,The First People′s Hospital of Kunming,Kunming Yunnan 650051,China)
出处
《蚌埠医学院学报》
CAS
2021年第12期1659-1663,共5页
Journal of Bengbu Medical College
作者简介
李志海(1974-),男,回族,主治医师.
