摘要
[目的]研究杨树AG类基因(PtAG1及PtAG2)在杨树雄花发育过程中的作用,为杨树分子育种提供基础。[方法]以‘84K’杨(Populus alba×P.glandulosa‘84K’)花芽为材料,对花芽发育过程连续采样,观察表型发育状况与测定PagAG2基因部位与表达量,通过石蜡切片、实时定量PCR、原位杂交等技术手段,对银腺杨‘84K’(Populus alba×P.glandulosa‘84K’)PagAG2基因时空特异性进行研究,分析PagAG2基因表达模式。[结果]在‘84K’杨雄花发育过程中,花药逐渐成熟,在此过程中,PagAG2的表达量先增加后降低,并且在‘84K’杨组培苗根、茎、叶中也能够检测到该转录物。原位杂交结果显示:PagAG2在‘84K’杨雄花花药中表达,但在包围花药的周围组织中不表达。[结论]‘84K’PagAG2基因与‘84K’杨雄花花发育密切相关,有望作为基因工程改良杨树花粉的目标基因用于研究。但PagAG2是否参与杨树其他组织的生长发育调控,在相关育种工作中需要对其做进一步的基因功能分析。
[Objective]To identify the role of PtAG2 gene in the development of male flowers of poplar.[Method]Based on the regulation of AG genes(PtAG1 and PtAG2)in the stamens and carpels of monoecious plants,the spatial and temporal specificity of PagAG2 gene of Populus alba×P.glandulosa‘84 K’was studied by means of paraffin section,real-time quantitative PCR and in situ hybridization.Taking the flower branches of P.alba×P.glandulosa‘84 K’as test materials,the inflorescences were continuously sampled within one week after hydroponic culture to observe the development of male flowers by paraffin sectioned technique.Real-time quantitative PCR was used to detect the expression of PagAG2 in the roots,stems,leaves and inflorescence of P.alba×P.glandulosa‘84 K’.In situ hybridization was used to detect the specific expression sites of PagAG2 in the flower organs of P.alba×P.glandulosa‘84 K’male flowers,so the expression pattern of PagAG2 gene was analyzed.[Result]During the development of male flowers of P.alba×P.glandulosa‘84 K’,the anthers matured gradually.At the same time,the expression of PagAG2 increased at initial and then decreased,and the transcription was also be detected in the roots,stems and leaves of tissue cultured P.alba×P.glandulosa‘84 K’.The results of in situ hybridization showed that PagAG2 was expressed in the anthers of P.alba×P.glandulosa‘84 K’male flowers but not in the surrounding tissues.[Conclusion]PagAG2 gene is closely related to the male flower development of P.alba×P.glandulosa‘84 K’,so it is expected to be a target gene for genetic engineering to improve poplar pollen.However,whether the PagAG2 gene participates in the regulation of the growth and development of other tissues of poplar needs to be further studied.
作者
钟姗辰
武舒
王黎
苏晓华
张冰玉
ZHONG Shan-chen;WU Shu;WANG Li;SU Xiao-hua;ZHANG Bing-yu(State Key Laboratory of Forest Genetics and Tree Breeding,Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)
出处
《林业科学研究》
CSCD
北大核心
2021年第6期73-80,共8页
Forest Research
基金
国家自然基金项目(31770710)
转基因生物新品种培育重大课题(2018ZX08020002)。
作者简介
通讯作者:张冰玉,博士,研究员。主要研究方向:林木遗传育种。电话:010-62889654,Email:byzhang@caf.ac.cn。
